Publications by authors named "Daniel L Fletcher"

The aim of this study was to compare antimicrobial resistance profiles of distinct types of Listeria monocytogenes isolated from a commercial poultry cooking plant. One hundred fifty-seven L. monocytogenes isolates representing 14 different actA types were tested for susceptibility to 19 antimicrobial drugs using the broth microdilution method.

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Salmonella is a major pathogen associated with poultry food products. Over the past 20 years, pressure to reduce human illness from poultry-related salmonellosis has resulted in intensive research activity as well as stronger regulatory standards in Europe, North America, and, because of international trade policies, throughout the world. In the United States, implementation of a Hazard Analysis Critical Control Point-based inspection program has been credited with reducing the incidence of Salmonella-positive carcasses from approximately 20 to 10%.

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Experiments were conducted to determine the ideal medium for detection of temperature abuse of fresh broiler chicken using impedance microbiological techniques. In three separate trials, 15 ready-to-cook broiler chicken carcasses were obtained from the chiller exit of three separate processing plants. Five carcasses were sampled immediately (day 0), 5 carcasses were sampled after temperature abusing at 25°C for 12 h and holding at 3°C for 6 days (temperature abused), and the remaining 5 carcasses were sampled after holding at 3°C for 7 days (day 7 controls).

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Broiler chicken carcasses were obtained from a commercial processing facility, rinsed with 100 ml of sterile brain heart infusion broth and the solutions held at 3, 4, 5, 6, or 9°C for 10 d. At 0, 7, and 10 d, total plate counts (TPC) and impedimetric measurements were conducted at incubation temperatures of 18 and 42°C. For plates incubated at 42°C, counts did not change after 7 d of storage when broiler carcass rinses were held at 3 and 4°C.

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