Publications by authors named "Daniel Komuczki"
Cysteine is considered an essential amino acid in the cultivation of Chinese hamster ovary (CHO) cells. An optimized cysteine supply during fed-batch cultivation supports the protein production capacity of recombinant CHO cell lines. However, we observed that CHO production cell lines seeded at low cell densities in chemically defined media enriched with cysteine greater than 2.
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- Chemically defined media are typically made in large batches and stored in hold tanks, but a new method allows for continuous on-demand reconstitution from dry powdered media.
- This method uses a hopper and screw conveyor to accurately feed amounts of the powdered media into a mixer over 12 hours, achieving results similar to traditional batch production.
- Both cell cultivation and detailed antibody analysis showed no significant differences in quality between the continuous and batch methods, suggesting the new approach could improve efficiency and reduce space requirements in production facilities.
The capture of recombinant antibodies from cell culture broth is the first critical step of downstream processing. We were able to develop a precipitation-based method for the capture and purification of monoclonal antibodies based on divalent cations, namely ZnCl. Traditional precipitation processes have to deal with high dilution factors especially for resolubilization and higher viscosity due to the use of PEG as precipitation or co-precipitation agent.
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