Electrospun Poly-ε-Caprolactone Nanofibers Incorporating Keratin Hydrolysates as Innovative Antioxidant Scaffolds.

This manuscript describes the development and characterization of electrospun nanofibers incorporating bioactive hydrolysates obtained from the microbial bioconversion of feathers, a highly available agro-industrial byproduct. The electrospun nanofibers were characterized using different instrumental methods, and their antioxidant properties and toxicological potential were evaluated. Keratin hydrolysates (KHs) produced by P45 were incorporated at 1, 2.

Enzymatic processing of animal by-products: production of antioxidant hydrolysates with Bacillus sp. CL18 crude protease.

Protein hydrolysates might display diverse bioactivities with potential relevance to human and animal health and food technology. Enzymatic hydrolysis of agro-industrial by-products is increasingly focused. In this study, a crude protease from Bacillus sp.

Unconventional microbial proteases as promising tools for the production of bioactive protein hydrolysates.

Enzymatic hydrolysis is the most prominent strategy to release bioactive peptides from different food proteins and protein-rich by-products. Unconventional microbial proteases (UMPs) have gaining increased attention for such purposes, particularly from the 2010s. In this review, we present and discuss aspects related to UMPs production, and their use to obtain bioactive protein hydrolysates.

Characterization and application of a crude bacterial protease to produce antioxidant hydrolysates from whey protein.

sp. CL14 crude protease was partially characterized and applied to obtain antioxidant whey protein isolate (WPI) hydrolysates. Optimal activity occurred at pH 9.

Remediation of soil contaminated with a commercial diesel-biodiesel blend (B12): A microcosm evaluation on the effects of (in)organic amendments.

Bioremediation of fuel-contaminated soils largely depends on microbial activities, which might be stimulated using (in)organic amendments. Attenuation of a diesel-biodiesel blend (B12) was investigated in microcosms during 93 days. Soil was spiked with B12 (5%, m m) and, in addition to contaminated Controls (unamended), soils received compost (COB), soybean hulls (SHB), or NPK fertilizer (IB) to reach a ~20:1 carbon-to-nitrogen (C:N) ratio regarding B12-carbon content.

Biochemical Properties of a Partially Purified Protease from Bacillus sp. CL18 and Its Use to Obtain Bioactive Soy Protein Hydrolysates.

Microbial proteases are relevant biocatalysts with diverse applications. Production of protein hydrolysates is recently focused, since they might display biological activities. Therefore, the extracellular protease from Bacillus sp.

Landfill leachate treatment by a boron-doped diamond-based photo-electro-Fenton system integrated with biological oxidation: A toxicity, genotoxicity and by products assessment.

A photo-electro-Fenton (PEF) reactor employing boron-doped diamond (BDD) and soft iron anodes was studied in landfill leachate (LL) treatment. The reactor operation parameters (ROP) HO concentration, current intensity and flow rate were investigated in the removal of Abs 254 nm. The PEF process with BDD anode, operating at the best operational conditions, was used as a pre-treatment and enabled biological oxidation (BO).

Beyond plucking: Feathers bioprocessing into valuable protein hydrolysates.

The livestock production and subsequent processing of meat results in huge quantities of solid waste such as viscera, bones, skin and keratin-rich materials, including feathers, hair, wool, claws and hooves. In particular, the continuous growth of poultry industry generates massive amounts of feathers as major waste material. The conversion of such by-products into materials with increased value has been studied.

Protease production by the keratinolytic Bacillus sp. CL18 through feather bioprocessing.

Bacillus sp. CL18 was investigated to propose a bioprocess for protease production using feathers as organic substrate. In feather broth (FB), containing feathers as sole organic substrate (1-100 g l), maximal protease production was observed at 30 g l (FB30) after 6 days of cultivation, whereas increased feather concentrations negatively affected protease production and feather degradation.

Antimicrobial activity of free and liposome-encapsulated thymol and carvacrol against Salmonella and Staphylococcus aureus adhered to stainless steel.

Antimicrobial activity of thymol, carvacrol and thymol/carvacrol liposomes (TCL) was evaluated against two bacterial pools, each one consisting of four strains of Staphylococcus aureus or Salmonella enterica. TCL were prepared using thin-film hydration, showing 270.20nm average diameter (polydispersity index of 0.

Soy protein hydrolysis with microbial protease to improve antioxidant and functional properties.

Soybean proteins are widely used as nutritional and functional food ingredients. This investigation evaluated through a 2(3) central composite design the effect of three variables (pH, temperature and enzyme/substrate (E/S) ratio) on the production of soy protein isolate (SPI) hydrolysates with a microbial protease. Soluble peptides, antioxidant activity, and foaming and emulsifying capabilities of the hydrolysates were analyzed.

Hydrolysates of sheep cheese whey as a source of bioactive peptides with antioxidant and angiotensin-converting enzyme inhibitory activities.

Enzymatic proteolysis may be employed to release bioactive peptides, which have been investigated for potential benefits from both technological and human health perspectives. In this study, sheep cheese whey (SCW) was hydrolyzed with a protease preparation from Bacillus sp. P7, and the hydrolysates were evaluated for antioxidant and angiotensin I-converting enzyme (ACE)-inhibitory activities.

Inhibition of Listeria monocytogenes in minas frescal cheese by free and nanovesicle-encapsulated nisin.

The effectiveness of free and nanovesicle-encapsulated nisin to control Listeria monocytogenes in Minas Frescal cheese was investigated. Commercial nisin was encapsulated into liposomes of partially purified soy lecithin. Free (0.

A current assessment on the production of bacterial keratinases.

Keratinolytic proteases are microbial enzymes that hydrolyze keratins, recalcitrant proteins found in the epidermis and epidermal appendages of vertebrates. Keratin-rich materials are abundantly generated as wastes from agroindustrial activities, particularly the meat and poultry industries. Therefore, these enzymes are postulated for several applications, including the bioconversion of keratin-rich materials, utilization as animal feed supplements, action as de-hairing agents in tannery, prion degradation, among others.

Kinetic modeling of thermal inactivation of the Bacillus sp. protease P7.

Data from thermal stability of a keratinolytic protease produced by the Amazon isolate Bacillus sp. P7 was fitted to various mathematical models. Kinetic modeling showed that Weibull distribution was the best equation to describe the residual activity of protease P7 after heat treatment.

Production of Proteolytic Enzymes by a Keratin-Degrading Aspergillus niger.

A fungal isolate with capability to grow in keratinous substrate as only source of carbon and nitrogen was identified as Aspergillus niger using the sequencing of the ITS region of the rDNA. This strain produced a slightly acid keratinase and an acid protease during cultivation in feather meal. The peak of keratinolytic activity occurred in 48 h and the maximum proteolytic activity in 96 h.

Kinetic stability modelling of keratinolytic protease P45: influence of temperature and metal ions.

The activity and kinetic stability of a keratinolytic subtilisin-like protease from Bacillus sp. P45 was investigated in 100 mM Tris-HCl buffer (pH 8.0; control) and in buffer with addition of Ca(2+) or Mg(2+) (1-10 mM), at different temperatures.

Production and properties of keratinolytic proteases from three novel Gram-negative feather-degrading bacteria isolated from Brazilian soils.

The keratinolytic potential and protease properties of three novel Gram-negative feather-degrading bacteria isolated from Brazilian soils was described. Aeromonas hydrophila K12, Chryseobacterium indologenes A22 and Serratia marcescens P3 were able to degrade feather meal, producing high amounts of soluble proteins and forming thiol groups. The proteases of strains K12, A22 and P3 had optimal pH of 8.

Effect of nanovesicle-encapsulated nisin on growth of Listeria monocytogenes in milk.

Commercial nisin was encapsulated in nanovesicles (mean diameter 140 nm) prepared from partially purified soy lecithin. Nisin-loaded liposomes and unencapsulated (free) nisin were initially tested in BHI medium and skim milk inoculated with Listeria monocytogenes and incubated for 48 h at 30 degrees C. At such abuse temperature conditions, free nisin showed better inhibitory than the liposomal counterparts.