Optimization Workflow of Fumonisin Esterase Production for Biocatalytic Degradation of Fumonisin B.

Industrial enzyme production with the expression system requires a well-characterized production strain and a competitively priced fermentation medium to meet the expectations of the industry. The present work shows a workflow that allows the rapid and reliable screening of transformants of single copy insertion of the target production cassette. A constitutive expression system with the glyceraldehyde-3-phosphate dehydrogenase promoter () with homology arms for the glycerol kinase 1 () was constructed for the targeted integration of the expression plasmid in a deficient and the production of a bacterial fumonisin esterase enzyme (CFE).