Publications by authors named "Daniel J Selski"
A customized retroviral vector confers marker gene expression in osteoclast lineage cells.
J Cell Biochem
February 2006
Osteoclasts play a seminal role in many skeletal diseases and therefore are candidates for cell-based gene delivery systems to treat disorders of bone. As an initial step toward developing osteoclast-mediated gene delivery systems, we have made and analyzed a customized Molony-Murine leukemia virus (MMLV)-based retroviral vector containing elements of the osteoclast-specific tartrate-resistant acid phosphatase (TRAP) gene. RAW 264.
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- The study investigates the tartrate-resistant acid phosphatase (TRAP) gene, which shows different expression patterns in various tissues, pointing to the existence of alternative mRNA transcripts driven by distinct promoters.
- Transgenic mice were created using two candidate promoters (BC and C); the BC promoter showed strong expression across multiple tissues, while the C promoter did not show significant expression in vivo or vitro.
- The findings indicate that the expression of bone- and spleen-preferred TRAP transcripts relies on regulatory elements beyond the BC and C promoters, highlighting the complexity of transcriptional regulation in osteoclast gene expression.