Publications by authors named "Daniel J Pritko"
Article Synopsis
- - Genetically-encoded single-cell barcodes can help with tasks like tracing cell lineages and conducting genetic screens, but current methods have limitations in speed and effectiveness.
- - This study proposes a new method using combinations of fluorescent proteins to create a high-diversity barcode library that allows for non-destructive, quick, and affordable identification of cells.
- - The researchers successfully tested this method, creating a library of about 150 unique barcodes using 18 fluorescent proteins, and demonstrated its effectiveness for classifying cells and enabling genetic screenings and lineage tracing.
Systematic, genome-scale genetic screens have been instrumental for elucidating genotype-phenotype relationships, but approaches for probing genetic interactions have been limited to at most ∼100 pre-selected gene combinations in mammalian cells. Here, we introduce a theory for high-throughput genetic interaction screens. The theory extends our recently developed Multiplexing using Spectral Imaging and Combinatorics (MuSIC) approach to propose ∼10 spectrally unique, genetically encoded MuSIC barcodes from 18 currently available fluorescent proteins.
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