Publications by authors named "Daniel J Nesbitt"
Cold exposure is an environmental stress that elicits a rapid metabolic shift in endotherms and is required for survival. The liver provides metabolic flexibility through its ability to rewire lipid metabolism to respond to an increased demand in energy for thermogenesis. We leveraged cold exposure to identify novel lipids contributing to energy homeostasis and found that lysosomal bis(monoacylglycero)phosphate (BMP) lipids were significantly increased in the liver during acute cold exposure.
View Article and Find Full Text PDFAldehydes are important synthons for DNA-encoded library (DEL) construction, but the development of a DNA-compatible method for the oxidation of alcohols to aldehydes remains a significant challenge in the field of DEL chemistry. We report that a copper/TEMPO catalyst system enables the solution-phase DNA-compatible oxidation of DNA-linked primary activated alcohols to aldehydes. The semiaqueous, room-temperature reaction conditions afford oxidation of benzylic, heterobenzylic, and allylic alcohols in high yield, with DNA compatibility verified by mass spectrometry, qPCR, Sanger sequencing, and ligation assays.
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- The study aimed to determine the rate of SARS-CoV-2 seropositivity among blood donors in Rhode Island, utilizing various antibody testing methods due to the lack of prior data on population seroconversion during the COVID-19 pandemic.
- Approximately 0.6% of blood donors tested positive for antibodies against SARS-CoV-2 during April-May 2020, coinciding with the peak of daily new COVID-19 cases in the region.
- The findings suggest that actual infection rates may be low within this population, and that certain antibody tests can effectively assess seroprevalence, indicating a need for further research using validated tests for more accurate results.
Article Synopsis
- - Projections for managing the SARS-CoV-2 pandemic rely on accurate measurements of serum neutralizing antibodies (NAb) using robust antibody assays, but current testing has led to confusion due to the variety of tests available.
- - A study involving 1,000 NYC blood donors in mid-2020 found that 12.1% and 10.9% were seropositive for antibodies using two different assays, indicating that around 1 in 8 donors showed signs of infection since the pandemic began.
- - The findings suggest a much lower actual infection rate than previously thought, contradicting the idea of achieving "herd immunity" at 60% or more, and highlight that not all individuals with antibodies
Development of a computerised decision aid for thrombolysis in acute stroke care.
BMC Med Inform Decis Mak
February 2015
Background: Thrombolytic treatment for acute ischaemic stroke improves prognosis, although there is a risk of bleeding complications leading to early death/severe disability. Benefit from thrombolysis is time dependent and treatment must be administered within 4.5 hours from onset of symptoms, which presents unique challenges for development of tools to support decision making and patient understanding about treatment.
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