Primary and memory immune responses against rough are less robust compared to smooth and following intratracheal infection in mice.

is the cause of canine brucellosis, a globally distributed, zoonotic pathogen which primarily causes disease in dogs. is unique amongst the zoonotic spp. with its rough lipopolysaccharide, a trait typically associated with attenuation in gram-negative bacteria.

Characterization of epididymal and testicular histologic lesions and use of immunohistochemistry and PCR on formalin-fixed tissues to detect in male dogs.

In male dogs, frequently causes epididymitis, ultimately resulting in testicular atrophy and infertility. Although predominantly affects the epididymis, the misleading term "orchitis" is still commonly used by clinicians. Of additional concern, diagnosis in dogs remains challenging because of variable sensitivity and specificity of serologic assays and fluctuations in bacteremia levels in infected dogs, reducing the sensitivity of blood culture.

Evaluation of the safety profile of the vaccine candidate Brucella melitensis 16MΔvjbR strain in goats.

Small ruminant brucellosis is caused by the Gram negative cocci-bacillus Brucella (B.) melitensis, the most virulent Brucella species for humans. In goats and sheep, middle to late-term gestation abortion, stillbirths and the delivery of weak infected offspring are the characteristic clinical signs of the disease.

Brucella abortus and Pregnancy in Mice: Impact of Chronic Infection on Fertility and the Role of Regulatory T Cells in Tissue Colonization.

Stealthy intracellular bacterial pathogens are known to establish persistent and sometimes lifelong infections. Some of these pathogens also have a tropism for the reproductive system, thereby increasing the risk of reproductive disease and infertility. To date, the pathogenic mechanism involved remains poorly understood.

Intratracheal Inoculation with Brucella melitensis in the Pregnant Guinea Pig Is an Improved Model for Reproductive Pathogenesis and Vaccine Studies.

Reproductive failure is the hallmark of brucellosis in animals. An uncommon but important complication in pregnant women who become acutely infected with is spontaneous pregnancy loss or vertical transmission to the fetus. Unfortunately, the mechanism behind reproductive failure is still obscure, partially due to the lack of a proper study model.

Evaluation of the Efficacy of the Brucella canis RM6/66 Δ Vaccine Candidate for Protection against B. canis Infection in Mice.

is a Gram-negative, facultative intracellular bacterium and the causative agent of canine brucellosis, a highly contagious disease of dogs that can be transmitted to humans. Unfortunately, no vaccine is available to prevent infection. We recently characterized the kinetics of infection in the mouse model, establishing the required dose necessary to achieve systemic infection.

Vaccine Candidate Brucella melitensis 16M Is Safe in a Pregnant Sheep Model and Confers Protection.

As a natural host species for , pregnant sheep offer an ideal model to evaluate vaccine candidates for safety. strain Rev. 1 has been used almost exclusively to prevent brucellosis in small ruminants, but it causes abortions when given to pregnant animals.

Interaction of Brucella abortus with Osteoclasts: a Step toward Understanding Osteoarticular Brucellosis and Vaccine Safety.

Osteoarticular disease is a frequent complication of human brucellosis. Vaccination remains a critical component of brucellosis control, but there are currently no vaccines for use in humans, and no models for assessing the safety of candidate vaccines in reference to the development of bone lesions currently exist. While the effect of infection on osteoblasts has been extensively evaluated, little is known about the consequences of osteoclast infection.

Brucellosis in Colombia: Current Status and Challenges in the Control of an Endemic Disease.

Brucellosis is a zoonosis of nearly worldwide distribution. The disease is considered to be endemic in most of the developing countries with a substantial impact on both human and animal health as well as on the economy. The aim of this scoping review is to provide an overview of the brucellosis status in Colombia and the factors associated with its persistence, to highlight the strengths and gaps of the adopted countermeasures and to supply evidence to policy-makers on the best approaches to mitigate the disease burden.

Vaccine safety studies of S19 and S19Δ in pregnant swine.

Brucellosis in swine is caused by a bacterial infection of nearly worldwide distribution. is also transmissible to humans, dogs and cattle and is considered a reemerging disease of public health concern. To date, there is no effective vaccine for swine.

Characterization of Brucella canis infection in mice.

Canine brucellosis, caused by Brucella canis, is a disease of dogs and represents a public health concern as it can be transmitted to humans. Canine brucellosis is on the rise in the United States and there is currently no vaccine for use in dogs. Mice have been extensively utilized to investigate host-pathogen interactions and vaccine candidates for smooth Brucella species and could serve a similar role for studying B.

Correction: Characterization of an intratracheal aerosol challenge model of Brucella melitensis in guinea pigs.

[This corrects the article DOI: 10.1371/journal.pone.

The NOD- Mouse Model Is Suitable for the Study of Osteoarticular Brucellosis and Vaccine Safety.

Osteoarticular brucellosis is the most common complication in -infected humans regardless of age, sex, or immune status. The mechanism of bone destruction caused by species remained partially unknown due to the lack of a suitable animal model. Here, to study this complication, we explored the suitability of the use of the NOD- mouse to study osteoarticular brucellosis and examined the potential use of this strain to evaluate the safety of live attenuated vaccine candidates.

Characterization of an intratracheal aerosol challenge model of Brucella melitensis in guinea pigs.

B. melitensis is considered the most virulent of the Brucella species, and a need exists for an improved laboratory animal model of infection that mimics natural transmission and disease. Guinea pigs are highly susceptible to infection with Brucella spp.

Human Brucellosis and Adverse Pregnancy Outcomes.

Purpose Of Review: Brucellosis is a neglected, zoonotic disease of nearly worldwide distribution. Despite brucellosis being recognized as a reproductive disease in animals, it has been historically known as a flu-like illness in humans with little or no significant role in maternal or newborn health. This review focuses on what is currently known relative to the epidemiology of brucellosis in human pregnancy as well as new insights of placental immunology.