The heat is on: a simple method to increase genome editing efficiency in plants.

Background: Precision genome mutagenesis using CRISPR/Cas has become the standard method to generate mutant plant lines. Several improvements have been made to increase mutagenesis efficiency, either through vector optimisation or the application of heat stress.

Results: Here, we present a simplified heat stress assay that can be completed in six days using commonly-available laboratory equipment.

Analysis of predictors of mortality after surgical and non-surgical management in proximal humerus fractures.

Background: Proximal humerus fractures are one of the main osteoporotic fractures. Choosing between conservative or surgical treatment is a controversial topic in the literature, as is the functional impact. The main aim of our study was to analyse whether patient comorbidities should influence the final therapeutic decision for these fractures.