The introduction of fish into mountain lakes typically leads to profound ecological changes within the food web, but its consequences depend on the dietary preferences of fish and on the resistance of prey organisms against predation. Here we used stable isotopes and fatty acid analyses in combination with the traditional stomach content analysis to examine the diet of an allopatric population of Salmo trutta, which has originally been stocked during the Middle Ages in an alpine lake, and to identify what components of the food web are more affected. The results from stable isotopes and fatty acids indicated that planktonic and benthic food sources, in particular chironomids larvae, were the most important prey items all year round.
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