Impact of elastic substrate on the dynamic heterogeneity of WC256 Walker carcinosarcoma cells.

Cellular heterogeneity is a phenomenon in which cell populations are composed of subpopulations that vary in their behavior. Heterogeneity is particularly pronounced in cancer cells and can affect the efficacy of oncological therapies. Previous studies have considered heterogeneity dynamics to be indicative of evolutionary changes within subpopulations; however, these studies do not consider the short-time morphological plasticity of cells.

Morphomigrational description as a new approach connecting cell's migration with its morphology.

The examination of morphology and migration of cells plays substantial role in understanding the cellular behaviour, being described by plethora of quantitative parameters and models. These descriptions, however, treat cell migration and morphology as independent properties of temporal cell state, while not taking into account their strong interdependence in adherent cells. Here we present the new and simple mathematical parameter called signed morphomigrational angle (sMM angle) that links cell geometry with translocation of cell centroid, considering them as one morphomigrational behaviour.

Design and Construction of a Chamber Enabling the Observation of Living Cells in the Field of a Constant Magnetic Force.

The aim of the work was to design and construct a microscopic stage that enables the observation of biological cells in a magnetic field with a constant magnetic force. Regarding the requirements for biological observations in the magnetic field, construction was based on the standard automatic stage of an optical microscope ZEISS Axio Observer, and the main challenge was to design a set of magnets which were the source of a field in which the magnetic force was constant in the observation zone. Another challenge was to design a magnet arrangement producing a weak magnetic field to manipulate the cells without harming them.

Thermal Deformations of Thermoplast during 3D Printing: Warping in the Case of ABS.

This research focuses on thermal deformations of thermoplast during three-dimensional printing. A filament acrylonitrile butadiene styrene was used, and the main focus was put on warping. Twenty-seven cuboids divided in six categories by their length, height, surface area, color, nozzle temperature and bed temperature were printed by Fused Filament Fabrication 3D printer.

Physics Doesn't Bite-A Simple Experiment for Introducing Biomechanical Operational Principles of the Temporomandibular Joint.

Biophysics is rarely mentioned as one of the most useful parts of dental and medical students' curricula. However, with the growing complexity of tools and methods used in diagnostics and therapy, the knowledge of their physical foundations becomes important and helps with choosing the optimal solutions for both, a patient and a doctor. The aim of the proposed activity is to develop students' intuition about simple physical models that help with understanding fundamental properties of temporomandibular joint (TMJ).

A simplified experimental method to study conductivity percolation.

A simplified experimental setup and a measurement method adapted to the simplified data analysis proposed in previous works are described in detail. The setup is intended for investigation of the conductivity percolation in the water network formed on granular materials and studied in the course of dehydration. The results show that the new, easily accessible experimental setup enables determination of the principal percolation parameters with the same accuracy as the more sophisticated equipment employing an impedance analyzer.

Effect of substrate elasticity on macroscopic parameters of fish keratocyte migration.

Cell migration is an important biological phenomenon which depends on a number of internal and external factors. One of such factors can be the mechanical properties of the environment which can have an impact on the cell's regulatory pathways through so-called mechanotransduction. Ultimately, these properties can also influence the process of cell migration.

Increasing microscopy resolution with photobleaching and intensity cumulant analysis.

Super-resolution fluorescence microscopy and its applications for analysis of biological structures are evolving rapidly field. A number of approaches aimed at overcoming the fundamental limit imposed by diffraction have been proposed in recent years. Here we present a modification of super-resolution optical fluctuation imaging (SOFI), a technique based on spatio-temporal evaluation of the optical signal from independently fluctuating emitters.

Electric conductivity percolation in naturally dehydrating, lightly wetted, hydrophilic fumed silica powder.

In studying the dehydration of surface-moistened fumed silica Aerosil powders, we found a conductivity percolation transition at low hydration levels. Both the percolation exponent and the threshold are typical for correlated site-bond transitions in complex two-dimensional (2D) systems. The exponent values, 0.