New protocol to affix sample for environmental and high vacuum electron microscopy.

Electron microscopy has become increasingly essential as an investigative research resource in various fields of science. Despite the evolution of the equipment and preparation techniques, little has been published on techniques for affixation and removal of samples on the stubs after analysis. This work aims to illustrate a quick and low-cost procedure for fixing and removing a sample from the support for analysis by electronic microscopy, applied to crustacean and insect species, as well as other invertebrates.

Three new records of Cladocera (Crustacea: Branchiopoda) for the State of Maranhão, Northeastern Brazil.

Brazilian biodiversity is still poorly studied, especially in the northeast region of Brazil. In the state of Maranhão, the rich fauna is still under-explored in relation to the Cladocera, and this geographic area covers four biomes, which makes it an important field of research on the Cladocera community. In this work, the record of three species is reported: Dadaya macrops Sars, 1901 first record of the genus for the state, Simocephalus serrulatus (Koch, 1841) and Simocephalus latirostris (Stingelin, 1906).