Background: Copper (Cu) is an essential microelement to the health and proper functioning of metabolic processes in animals, but the particular function of Cu in fermentation processes and the formation of methane (CH ) in the rumen have been poorly analyzed. The innovative aspect of this study was to investigate the effects of high doses of Cu as copper sulfate on in vitro ruminal degradation, fermentation patterns, and CH production.
Results: There was a decrease (P < 0.