Publications by authors named "Daniel Bertin"
Despite its wide use to treat various inflammatory diseases, infliximab becomes ineffective in some patients due to inadequate drug levels and production of anti-drug antibodies (ADA). The aim of this study was to compare the prevalence and ADA levels in a large cohort of patients. ADA and infliximab (IFX) through levels measured by enzyme-linked immunosorbent assay were collected from 505 patients within a period of 4 years.
View Article and Find Full Text PDFArticle Synopsis
- The study investigates the presence and importance of autoantibodies against lysobisphosphatidic acid (aLBPA) in patients with antiphospholipid syndrome (APS).
- It compares 91 patients with antiphospholipid antibodies—60 symptomatic and 31 asymptomatic—to 33 controls, finding a higher prevalence of aLBPA among patients.
- The research suggests that testing for aLBPA alongside conventional antiphospholipid antibodies may help in managing APS, especially in deciding if asymptomatic patients should receive preventive treatment.
Article Synopsis
- Apheresis is a medical procedure used to quickly remove harmful antibodies in diseases like anti-GBM and severe ANCA-associated vasculitis, and the CINEVAS study compared two methods: immunoadsorption (IA) and plasma exchange (PEx).
- The study analyzed results from 38 patients and found that both IA and PEx effectively reduced autoantibody levels similarly, with 98% reduction for IA and 96% for PEx after seven sessions.
- While IA showed a greater reduction in total IgG and better preservation of IgA and IgM, the volume of plasma used and session lengths differed between the techniques, but overall, their effectiveness in removing antibodies was comparable.
Objectives: This review aims to identify biological markers associated with the risk of recurrence of thrombotic and/or obstetric events in patients with antiphospholipid syndrome (APS).
Methods: A comprehensive review of literature was conducted to evaluate established and potential novel biological markers associated with thrombosis in APS. To this end, a PubMed literature search was conducted for the last twenty years using the following keywords or their combinations: thrombotic risk, recurrence of thrombosis, risk stratification, severity, predictive value.
Article Synopsis
- The study investigates the role of anti-RNP autoantibodies in diagnosing mixed connective tissue disease (MCTD) and distinguishing it from systemic lupus erythematosus (SLE).
- Researchers analyzed samples from 74 patients with anti-RNP autoantibodies, identifying that the ratio of autoantibodies targeting the U1-snRNP complex was significantly higher in MCTD patients compared to those with SLE.
- Findings suggest that assessing the overall RNP index is a more reliable diagnostic tool than evaluating individual autoantibodies, with potential implications for predicting disease progression.
The success of artificial intelligence and machine learning is an incentive to develop new algorithms to increase the rapidity and reliability of medical diagnosis. Here we compared different strategies aimed at processing microscope images used to detect anti-neutrophil cytoplasmic antibodies, an important vasculitis marker: (i) basic classifier methods (logistic regression, k-nearest neighbors and decision tree) were used to process custom-made indices derived from immunofluorescence images yielded by 137 sera. (ii) These methods were combined with dimensional reduction to analyze 1733 individual cell images.
View Article and Find Full Text PDFBackground: Anti-Jo-1 autoantibodies represent essential markers in the diagnosis of antisynthetase syndrome (ASS). In this retrospective study, we aimed to investigate whether their concentrations and fluctuations could both respectively reflect the severity and evolution of ASS.
Methods: Between 2015 and 2020, clinical and biological features of ASS patients with at least one positive measure of anti-Jo-1 autoantibody were collected.
Whereas the detection of antiphospholipid autoantibodies (aPL) in COVID-19 is of increasing interest, their role is still unclear. We analyzed a large aPL panel in 157 patients with COVID-19 according to the disease severity. We also investigated a potential association between aPL and extracellular DNA (exDNA, n = 85) or circulating markers of neutrophil extracellular traps (NET) such as citrullinated histones H3 (CitH3, n = 49).
View Article and Find Full Text PDFArticle Synopsis
- A new test for detecting anti-phosphatidylethanolamine autoantibodies (aPEs) could help doctors better diagnose and treat patients with antiphospholipid syndrome (APS), especially when other tests come back negative.
- Researchers studied 1,131 patients and found that 19.5% had aPEs, which was useful for those with unexplained deep vein thrombosis (DVT).
- They discovered a connection between having aPEs and increased production of reactive oxygen species (ROS), which points to a new way that aPEs might cause problems like blood clots.
Article Synopsis
- Hypocomplementemic urticarial vasculitis (HUV) is a rare disease that can affect the kidneys, and this study looked at how it does that in patients from multiple centers in France.
- Out of 12 patients studied, many had serious kidney issues, like protein in their urine and some needed temporary dialysis.
- After almost 9 years of follow-up, some patients had healthy kidney function, but others faced serious kidney problems, including needing a kidney transplant.
Persistence of various symptoms in patients who have recovered from coronavirus disease 2019 (COVID-19) was recently defined as 'long COVID' or 'post-COVID syndrome' (PCS). This article reports a case of a 58-year-old woman who, although recovering from COVID-19, had novel and persistent symptoms including neurological complications that could not be explained by any cause other than PCS. In addition to a low inflammatory response, persistence of immunoglobulin G anticardiolipin autoantibody positivity and eosinopenia were found 1 year after acute COVID-19 infection, both of which have been defined previously as independent factors associated with the severity of COVID-19.
View Article and Find Full Text PDFBackground: Although the triple positivity of antiphospholipid antibodies (aPL) is important for classifying high-risk patients, interpretation of aPL positivity, namely the lupus anticoagulant (LA), anti-cardiolipin (aCL), and anti-beta2-glycoprotein I autoantibodies (aB2GPI) remains challenging for thrombotic risk stratification.
Objective: To compare biological and clinical data between triple aPL- and single aCL-positive patients.
Methods: Of the 6500 patients assayed for aPL in daily practice within 3 years, we retrospectively analyzed data from 161 patients that were either triple aPL-positive or single aCL-positive with 5 years' follow-up for 121 of them.
Considering the level of myositis-specific autoantibodies could improve the precision of multiplex assay : lesson from patients with multiple positive results.
Semin Arthritis Rheum
February 2022
Background: The detection of additional autoantibodies is of great concern in systemic sclerosis (SSc) when those included in the ACR/EULAR classification are negative. In this context, the interest of antifibrillarin (anti-U3RNP) autoantibodies (AFAs) in the routine evaluation of SSc remains unclear. We aimed to assess the relevance of AFAs and their clinical association in SSc patients.
View Article and Find Full Text PDFObjective: To determine the clinical significance of anti-nuclear mitotic apparatus (NuMA) antibodies (AC-26 or AC-25) in patients with primary Sjögren's syndrome (pSS) and SLE.
Methods: Between 2013 and 2018, clinical and immunological features of pSS and SLE patients with anti-NuMA antibodies were compared with anti-NuMA antibodies-negative pSS and SLE cohorts.
Results: Among 31 284 sera positive for antinuclear antibodies, 90 patients (0.
Background: Inflammatory myopathies (IM) are characterized by muscular inflammation that can be associated with systemic disorders including lung. Anti-NXP2 antibody (Ab) is a rare myositis-specific antibody and its association with pulmonary involvement is still unknown. In this study, we investigated the characteristics of lung disease in patients with IM associated with anti-NXP2 Ab.
View Article and Find Full Text PDFThromboses in tuberculosis are linked to antiphosphatidylethanolamine antibodies levels: A cross-sectional study.
J Clin Tuberc Other Mycobact Dis
May 2019
Venous thromboses have been associated with tuberculosis, but the relationship with circulating anticoagulant has not been studied yet. In a cohort of 48 patients with tuberculosis, 22.9% of them presented with venous thromboses significantly associated with dose dependent level of antiphosphophatidyl-ethanolamine antibodies.
View Article and Find Full Text PDFObjectives: Non-conventional aPL have been described in patients presenting clinical manifestations of antiphospholipid syndrome but negative for conventional markers. Among them, detection of autoantibodies against prothrombin has been proposed to improve diagnosis and management of these patients. However autoantibodies against prothrombin are heterogeneous and their use in clinical practice still remains unclear.
View Article and Find Full Text PDFArticle Synopsis
- - A study was conducted to compare the performance of three ELISA kits used for measuring infliximab serum trough levels (s-IFXt) and anti-drug antibodies (ADAb) in 35 patients with inflammatory bowel disease (IBD).
- - Key differences in technical characteristics of the kits were found, impacting the measurement results; Ridascreen® reported s-IFXt levels significantly higher than the other two kits, leading to discrepancies in patient classification.
- - The findings indicate that the assays are not interchangeable for quantitative results, emphasizing the need for consistency in the assay used for monitoring IBD patients to avoid conflicting treatment decisions.
Article Synopsis
- The study presents the first pediatric case of auto-immune hepatitis with detectable anti-P antibodies.
- This case highlights the potential need for testing for auto anti-P antibodies in children with seronegative hepatitis autoimmune injury (HAI).
- The recommendation suggests that screening for these antibodies could improve diagnosis and understanding of pediatric auto-immune conditions.
Objectives: We aimed to assess the clinical significance of Krebs von den Lungen-6 (KL-6) in the diagnosis and severity of interstitial lung disease (ILD) in a French cohort of patients with systemic sclerosis (SSc).
Methods: Serum KL-6 concentrations were measured with chemiluminescent enzyme immunoassay (CLEIA) in 75 SSc patients. Patients were divided into two groups according to the presence of interstitial lung disease (SSc-ILD versus SSc-without ILD) on chest High-Resolution Computed Tomography.