Publications by authors named "Daniel B Hatchett"
Extracellular Vesicles (EVs) are membrane-derived vesicles shed by cells into the extracellular space that play key roles in intercellular communication and other biological processes. As membrane-bound cargos of nucleic acids and other proteins that are abundantly found in virtually every biofluid including blood, urine, and saliva, EVs are widely regarded as promising biomarkers for disease detection. While it is an increasingly promising biofluid from which to isolate EVs, saliva poses challenges due its complexity and heterogeneity-cells, debris, and other proteins can inhibit the isolation of EVs by traditional platforms.
View Article and Find Full Text PDFThe CandyCollect device is a lollipop-inspired open fluidic oral sampling device designed to provide a comfortable user sampling experience. We demonstrate that the CandyCollect device can be coupled with a rapid antigen detection test (RADT) kit designed for Group A Streptococcus (GAS). Through experiments with pooled saliva spiked with we tested various reagents and elution volumes to optimize the RADT readout from CandyCollect device samples.
View Article and Find Full Text PDFArticle Synopsis
- Traditional pharyngeal swabs are uncomfortable for children, leading to issues like reluctance to visit the clinic and potentially incorrect diagnoses for sore throats.
- The CandyCollect device, a lollipop-inspired sampling tool, effectively captured Group A Streptococcus in children, with high approval ratings from both children and caregivers regarding its taste and ease of use.
- While all tested methods (CandyCollect, mouth swab, and pharyngeal swab) were equally effective, there is potential for improvement in the collection time for CandyCollect without compromising its appealing design.