Identification of products of the uridinediphospho-N-acetyl-D-glucosamine oxidoreductase system from Citrobacter freundii ATCC 10053.

Uridinediphospho-N-acetyl glucosamine is converted to one or more uridine-diphospho-4-keto-6-deoxy-2-acetamidohexose derivatives by an enzyme from Citrobacter freundii ATCC 10053. Borohydride reduction of reaction products followed by acid hydrolysis yielded several amino sugars. Two of these were identified as fucosamine and quinovosamine by chromatography and ninhydrin degradation.

Release of neurohypophysial hormones in vitro.

1. The rate of release of neurohypophysial hormones in vitro, using isolated, halved neural lobes of the rat in an incubation medium containing excess K(+) and Ca(2+), was measured. The highest average rate of release was observed between 10 and 20 min after commencement of incubation.