Publications by authors named "Daniel A Carrillo-Vazquez"
Systemic lupus erythematosus (SLE) is an autoimmune disorder that often leads to kidney injury, known as lupus nephritis (LN). Although renal biopsy is the primary way to diagnose LN, it is invasive and not practical for regular monitoring. As an alternative, several groups have proposed urinary extracellular vesicles (uEVs) as potential biomarkers for LN, as recent studies have shown their significance in reflecting kidney-related diseases.
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- The study investigates how the activity of systemic lupus erythematosus (SLE) affects the body's innate immune response by comparing SLE patients to healthy donors.
- Researchers evaluated patients during disease flare-ups and after treatment, focusing on immune cell behaviors and markers through various laboratory techniques.
- Findings indicate that higher disease activity correlates with altered immune cell functions, suggesting that disease severity affects patients' risk for infections and overall immune response.
Introduction: Immunoglobulin A (IgA) is the main antibody isotype in body fluids such as tears, intestinal mucous, colostrum, and saliva. There are two subtypes of IgA in humans: IgA1, mainly present in blood and mucosal sites, and IgA2, preferentially expressed in mucosal sites like the colon. In clinical practice, immunoglobulins are typically measured in venous or capillary blood; however, alternative samples, including saliva, are now being considered, given their non-invasive and easy collection nature.
View Article and Find Full Text PDFLupus nephritis (LN) is one of the most common manifestations of systemic lupus erythematosus (SLE), characterized by abnormal B cell activation and differentiation to memory or plasma effector cells. However, the role of these cells in the pathogenesis of LN is not fully understood, as well as the effect of induction therapy on B cell subsets, possibly associated with this manifestation, like aged-associated B cells (ABCs). Consequently, we analyzed the molecules defining the ABCs subpopulation (CD11c, T-bet, and CD21) through flow cytometry of blood samples from patients with lupus presenting or not LN, following up a small sub-cohort after six months of induction therapy.
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- COVID-19 is linked to increased production of neutrophil extracellular traps (NETs) and associated autoimmune responses, particularly in severe cases.
- The study found that patients with severe/critical COVID-19 had a higher proportion of low-density granulocytes (LDGs) and reduced capacity to degrade NETs, which were connected to the severity of the disease and inflammatory markers.
- Anti-NET antibodies were identified in COVID-19 patients, correlating with the presence of other autoantibodies, indicating that NETs may drive both inflammation and potential autoimmune reactions.
Background/objective: Biomarkers for disease activity and damage accrual in idiopathic inflammatory myopathies (IIMs) are currently lacking. The purpose of this cross-sectional study is to analyze the relationship among low-density granulocytes (LDGs), neutrophil extracellular traps (NETs), and clinical and immunological features of patients with IIM.
Methods: We assessed disease activity, damage accrual, amount of LDGs, NETs, expression of LL-37, and serum cytokines in 65 adult patients with IIM.
Most COVID-19 mortality scores were developed at the beginning of the pandemic and clinicians now have more experience and evidence-based interventions. Therefore, we hypothesized that the predictive performance of COVID-19 mortality scores is now lower than originally reported. We aimed to prospectively evaluate the current predictive accuracy of six COVID-19 scores and compared it with the accuracy of clinical gestalt predictions.
View Article and Find Full Text PDFConformational changes in myeloperoxidase induced by ubiquitin and NETs containing free ISG15 from systemic lupus erythematosus patients promote a pro-inflammatory cytokine response in CD4 T cells.
J Transl Med
November 2020
Background: Neutrophil extracellular traps (NETs) from patients with systemic lupus erythematosus (SLE) are characterized by lower ubiquitylation and myeloperoxidase (MPO) as a substrate. The structural and functional effect of such modification and if there are additional post-translational modifications (PTMs) are unknown.
Methods: To assess the expression and functional role of PTMs in NETs of patients with SLE; reactivation, proliferation and cytokine production was evaluated by flow cytometry using co-cultures with dendritic cells (DC) and CD4 from SLE patients and healthy controls.
TLR expression in peripheral monocyte subsets of patients with idiopathic inflammatory myopathies: association with clinical and immunological features.
J Transl Med
March 2020
Article Synopsis
- Monocytes and toll-like receptors (TLR) play a significant role in the inflammation seen in idiopathic inflammatory myopathies (IIM), but their specific subsets and TLR expression have not been fully explored.
- A study involving 45 IIM patients and 15 healthy controls assessed monocyte subsets, TLR expression, disease activity, and related health parameters using flow cytometry and other evaluations.
- Findings revealed that IIM patients had increased intermediate monocytes and TLR4 expression, with various correlations between TLR expression and clinical characteristics, such as dysphagia and interstitial lung disease.
Objectives: To analyse the potential contribution of low-density granulocytes (LDGs) and NETosis, as well as the differential protein cargo of neutrophil extracellular traps (NETs), as physiopathogenic mechanisms of adult-onset Still's disease (AOSD).
Methods: We recruited 30 patients with AOSD according to the Yamaguchi diagnostic criteria. LDGs were addressed by multiparametric flow cytometry as those CD14-, CD15+, CD10+ cells in the peripheral blood mononuclear cells fraction.