PLGA nanocarriers biomimetic of platelet membranes and their interactions with the placental barrier.

This study focuses on the preparation and characterization of platelet membrane biomimetic nanocarriers (P-PLGA NPs) and investigates their interactions with the transplacental barrier. Poly (lactic-co-glycolic acid) nanoparticles (PLGA NPs) were coated with platelet membrane (PLTM) to construct P-PLGA NPs. Additionally, fluorinated polyethylenimine (F-PEI) was grafted onto PLGA NPs to prepare F-PEI-PLGA NPs, which were compared with PLGA NPs.

Moxibustion inhibits the macrophage M1 polarization toll-like receptor 4/myeloid differentiation factor 88/nuclear factor kappa B signaling pathway by regulating T-cell immunoglobulin and mucin-containing protein-3 in rheumatoid arthritis.

Objective: To explore whether moxibustion exerts therapeutic effects on rheumatoid arthritis (RA) by regulating the expression of T-cell immunoglobulin and mucin-containing protein-3 (TIM-3) and subsequently modulating the macrophage M1 polarization toll-like receptor 4 (TLR4)-myeloid differentiation factor 88 (MyD88)-nuclear factor kappa B (NF-κB) signaling pathway.

Methods: We utilized moxibustion treatment in RA rat models using the Zusanli (ST36) and Shenshu (BL23) acupoints. Hematoxylin and eosin (HE) staining was used to observe the pathological changes of the synovial tissue under a section light microscope, and pathological scoring was performed according to the grading standard of the degree of synovial tissue disease.

Moxibustion of Zusanli (ST36) and Shenshu (BL23) alleviates the inflammation of rheumatoid arthritis in rats through regulating macrophage migration inhibitory factor/glucocorticoids signaling.

Objective: To test the hypothesis that moxibustion may inhibit rheumatoid arthritis (RA) synovial inflammation by regulating the expression of macrophage migration inhibitory factor (MIF)/glucocorticoids (GCs).

Methods: Fifty male Sprague-Dawley rats were randomly divided into five groups ( 10 each): blank Control (CON) group, RA Model (RA) group, Moxibustion (MOX) group, MIF inhibitor (S,R)-3-(4-hydroxyphenyl)-4,5-dihydro-5-isoxazole acetic acid methyl ester (ISO-1) group, and Moxibustion + MIF inhibitor ISO-1 (MOX + ISO-1) group. Rats in the ISO-1 group and ISO-1 + MOX group were intraperitoneally injected with the inhibitor ISO-1.

[miR-25-3p down-regulates the expression of ADAM10 to inhibit the differentiation of P19 cells into cardiomyocytes by blocking the Notch signaling pathway].

Objective To investigate the effect of miR-25-3p targeting a disintegrin and metalloproteinase 10 (ADAM10) on the differentiation of P19 cells into cardiomyocytes by regulating Notch signaling pathway. Methods P19 cells were induced to differentiate into cardiomyocytes with dimethyl sulfoxide (DMSO) and collected at 0, 5, and 10 days. The mRNA levels of myocardial differentiation markers GATA4, cTnT, atrial natriuretic polypeptide (ANP) and the level of miR-25-3p were detected by real-time quantitative PCR (qRT-PCR).

[Pyroptosis is involved in the pathogenesis of necrotizing enterocolitis].

Objective To investigate the role of pyroptosis in the pathogenesis of necrotizing enterocolitis (NEC). Methods Sixty SD rats aged one day were divided into normal control group (n=30) and NEC group (n=30). The NEC model was established in the NEC group by hypoxia and cold stimulation, and the control group was not treated.