Reproductive outcome in globozoospermic men: update and prospects.

Infertility affects approximately 15% of couples in reproductive age. Male infertility is estimated to represent about 20% of the etiologies. Among them, a rare type of teratozoospermia known as globozoospermia leads to disappointing pregnancy outcomes.

Restriction fragment length polymorphism of the human C3 complement gene.

A cloned gene-specific probe for human complement (C3) was hybridized to DNA samples digested with various restriction endonucleases. The C3 probe detects one restriction fragment length polymorphism (RFLP) that occurs frequently in the French population when DNAs are digested with Sac I. The corresponding DNA alleles can be readily used in linkage analysis of loci on chromosome 19, and such a polymorphism can be followed through myotonic dystrophy families.

[Restriction polymorphism of 3 human genomic probes on the short arm of the X chromosome in a reference population, and applications in prenatal and carrier female diagnosis of Duchenne's muscular dystrophy].

Restriction polymorphisms of three human genomic probes (RC8, L1-28 and p754), located on the short arm of the X chromosome, are studied in a French population panel after digestion with taq I and Pst I endonucleases. Informativeness of these three probes in that population are, respectively, 0.18, 0.

[Establishment of a DNA bank of human origin].

High molecular weight DNA extracted from 50 unrelated parisian individuals constitutes a "DNA-thèque" which can be used at the population level. This procedure allows preparation from a variety of tissues such as liver, placenta and leucocytes. The "quality" of the DNA obtained (about 50 Kb, RNA and protein free) and the efficiency of the method (in terms of the yield of DNA obtained) are considered.

[Electrophoretic polymorphism in Erythrocebus patas].

Variations were observed at the prealbumin-2, carbonic anhydrase-I, 6-phosphogluconate dehydrogenase, phosphoglucomutase-II and adenylate kinase loci in Erythrocebus patas subspecies pyrrhonotus. But the level of heterozygosity was very low compared with those of the different African monkey species studied by the same electrophoretic analysis. The reasons for this low variation are discussed with regard to ethological and genetic structure characteristics of the group studied.