The molecular mechanism of gemcitabine in inhibiting the HIF-1α/VEGFB/FGF2/FGFR1 signaling pathway for ovarian cancer treatment.

Ovarian cancer is a common malignant tumor in women, exhibiting a certain sensitivity to chemotherapy drugs like gemcitabine (GEM). This study, through the analysis of ovarian cancer single-cell RNA sequencing (scRNA-seq) data and transcriptome data post-GEM treatment, identifies the pivotal role of hypoxia-inducible factor 1 alpha (HIF-1α) in regulating the treatment process. The results reveal that HIF-1α modulates the expression of VEGF-B, thereby inhibiting the fibroblast growth factor 2 (FGF2)/FGFR1 signaling pathway and impacting tumor formation.

Probabilistic Representation Differences between Decisions from Description and Decisions from Experience.

For mathematically identical risky decisions, different choices can be made depending on whether information about outcomes and their probabilities is learned by description or by experience, known as the description-experience gap. However, it is unclear whether different ways of obtaining information lead to different representation forms of probability, resulting in a description-experience gap. The current study investigates the representation formats of the alternative options' probability for decisions from description and decisions from experience.

Prediction model of no-response before the first transarterial chemoembolization for hepatocellular carcinoma: TACF score.

Previous clinic models for patients with hepatocellular carcinoma (HCC) receiving transarterial chemoembolization (TACE) mainly focused on the overall survival, whereas a simple-to-use tool for predicting the response to the first TACE and the management of risk classification before TACE are lacking. Our aim was to develop a scoring system calculated manually for these patients. A total of 437 patients with hepatocellular carcinoma (HCC) who underwent TACE treatment were carefully selected for analysis.

Conflict detection and base-rate extremity.

People tend to ignore the probabilistic rules cued by the base-rate information and rely on the heuristic intuition cued by the descriptive information to make "stereotypical" responses in base-rate problems. Conflict detection studies have shown that reasoners can detect conflicts between heuristic intuition and probabilistic considerations despite ultimately stereotypical responses. However, these studies primarily used extreme base-rate tasks.

Conflict Detection in Moderate Base-Rate Tasks: A Multi-Measure Study.

Empirical studies have found that although humans often rely on heuristic intuition to make stereotypical judgments during extreme base-rate tasks, they can at least detect conflicts between stereotypical and base-rate responses, which supports the dual-processing view of flawless conflict detection. The current study combines the conflict detection paradigm with moderate base-rate tasks of different scales to test the generalization and boundaries of flawless conflict detection. After controlling for possible confounding by the "storage failure" factor, the conflict detection results indicated that reasoners providing stereotypical heuristic responses to conflict problems were slower to respond, less confident in their stereotypical responses, and slower to indicate their reduced confidence than reasoners who answered no-conflict problems.

microRNA‑577 inhibits cell proliferation and invasion in non‑small cell lung cancer by directly targeting homeobox A1.

An increasing number of studies have indicated that the dysregulation of microRNAs (miRNAs/miR) is closely associated with non‑small cell lung cancer (NSCLC) development and progression by acting as tumor suppressors or oncogenes. Therefore, an in‑depth understanding of the biological roles of miRNAs in NSCLC may provide novel therapeutic methods for the treatment of patients with this disease. In the present study, reverse transcription‑quantitative polymerase chain reaction was used to detect miR‑577 expression in NSCLC tissues and cell lines.

Role of miR-214 in modulating proliferation and invasion of human colon cancer SW620 cells.

This study investigated the role of miR-214 in modulating proliferation and invasion of human colon cancer SW620 cells. Fifty-five patients with colon cancer who were treated in China-Japan Union Hospital of Jilin University from March 2014 to March 2015 were enrolled into this study. Their cancer and corresponding paracancerous tissues were collected and the expression levels of miR-214 were determined by RT-qPCR.

[Determination of 53 pesticide residues in tea by gas chromatography-tandem mass spectrometry and double internal standard method].

A gas chromatography-triple quadrupole mass spectrometry (GC-MS/MS) method with tandem double columns was established for the determination of 53 pesticide residues in tea. The sample was extracted by acetonitrile with buffering salts of QuEChERS method, cleaned up with an ENVI-Carb/NH solid-phase extractant, and determined by GC-MS/MS. To reduce the matrix effect in GC-MS/MS, gulonic acid lactone and sorbitol were added to the standard solutions as protectants; additionally, anthraquione-D8 and triphenyl phosphate were used as double internal standards.

Effect of honokiol on exotoxin proteins listeriolysin O and p60 secreted by Listeria monocytogenes.

Listeria monocytogenes is considered one of the most important foodborne pathogens. The virulence-related proteins listeriolysin O (LLO) and p60 are critical factors involved in Listeria pathogenesis. In the present study, we investigated the effect of honokiol on LLO and p60 secreted from L.

A G-quadruplex based label-free fluorescent biosensor for lead ion.

Many Pb(2+) biosensors based on Pb(2+)-specific RNA-cleaving DNAzyme have been developed in the past years. However, many of them have limited practical use because of high cost (e.g.

A sensitive and selective DNAzyme-based flow cytometric method for detecting Pb2+ ions.

A sensitive and selective Pb(2+) sensor based on GR-5 DNAzyme has been developed by a flow cytometric method.

An ultrasensitive electrochemical sensor for the mercuric ion via controlled assembly of SWCNTs.

An ultrasensitive "turn-on" electrochemical sensor for the Hg(2+) ion was proposed based on the T-Hg(2+)-T coordination chemistry and the controlled assembly of SWCNTs on the MHA/SAM-modified gold electrode.

Label-free fluorescent sensor for mercury(II) ion by using carbon nanotubes to reduce background signal.

A simple, selective and sensitive turn-on fluorescent sensor for the detection of mercury(II) ion was developed using Sybr Green I as the signal reporter and SWCNTs as the quencher. Due to the affinity of SWCNTs towards ssDNA and organic dye, Sybr Green I, thymine-rich ssDNA and SWCNTs could form a self-assembly of three components, resulting in fluorescence quenching. Upon addition of another thymine-rich ssDNA and mercury(II) ion, formation of dsDNA via T-Hg(2+)-T base pairs enabled Sybr Green I to intercalate into the dsDNA, resulting in the restoration of fluorescence.