Molecular identification of novel duck associated Chapparvovirus in ducks, first report from China.

Duck Associated Chapparvovirus (DaChPV) is a newly discovered virus within the Chaphamparvovirus genus, and first identified from Canadian wild ducks in 2021. In this study, DaChPV DNA was detected in 14 out of 137 tissues samples collected from diarrhea ducks across various provinces in China. Subsequently, eight complete genome sequences were amplified using overlapping primers and sequenced.

Insight into the effect of wheatgrass powder on steamed bread properties: Impacts on gluten polymerization and starch gelatinization behavior.

This study investigates the use of wheatgrass powder (WGP) as a functional ingredient in steamed bread, focusing on its effects on the nutritional composition and key biological macromolecules, specifically gluten and starch. Wheatgrass harvested at 8 days demonstrated optimal bioactive content, enhancing steamed bread quality. The incorporation of WGP (2.

Aging aggravates cognitive dysfunction in spontaneously hypertensive rats by inducing cerebral microvascular endothelial dysfunction.

Hypertension in the elderly can seriously lead to cerebral microvascular damage and promote the development of vascular cognitive impairment. While endothelial function is crucial in cerebral microvascular protection, it is unclear whether aging exacerbates hypertension-induced cognitive dysfunction through endothelial dysfunction. In this study, we injected D-galactose (D-gal) into 24 spontaneous hypertension rats (SHR) and 24 Wistar-Kyoto rats (WKY) for 12 weeks to induce aging.

Exosome-mediated effects of BRCA1 on cardiovascular artery disease.

The progression of coronary artery disease atherosclerosis (CAD) is closely associated with cardiomyocyte apoptosis and inflammatory responses. This study focused on investigating the impact of BRCA1 in exosomes (Exo) derived from M1 macrophages on CAD. Through the analysis of single-cell RNA-seq datasets, significant communication between macrophages and cardiomyocytes in CAD patients was observed.

Moderate electric field-stimulated brown rice germination: Insights into membrane permeability modulation and antioxidant system activation.

This study investigated the effects of moderate electric fields (MEF) on the germination of brown rice (BR) and the underlying mechanisms, focusing on membrane permeability and the antioxidant system. The results revealed a significant increase in germination rate, from 62 % to 84 %, at the 12th hour after exposure to a 100-V MEF. This enhancement was attributed to an increase in cell membrane permeability, a crucial factor in MEF-induced germination.

National cohort study on cardiometabolic index and incident stroke in middle-aged and older adults in China.

Unlabelled: Background The Cardiometabolic Index (CMI), a novel marker integrating central obesity and lipid metabolism, serves as a comprehensive indicator of metabolic health. While CMI has been linked to metabolic disorders like diabetes and cardiovascular diseases, its role in predicting stroke risk remains unclear. This study examines the association between CMI and stroke risk in Chinese adults aged 45 and older, highlighting its potential as a predictive tool for public health.

Characteristic profiles of molecular types, antibiotic resistance, antibiotic resistance genes, and virulence genes of isolates from caprine mastitis in China.

is a significant pathogen in dairy animals, particularly when it infects the mammary gland; however, its prevalence among dairy goats in China remains poorly understood. This study aimed to investigate the distribution and characteristics of isolates in dairy goats across China. A total of 515 milk samples were collected from goats diagnosed with mastitis in 14 provinces.

Phage-host interaction in clinical isolates with functional and altered quorum sensing systems.

Quorum sensing (QS) plays a crucial role in regulating key traits, including the upregulation of phage receptors, which leads to heightened phage susceptibility in . As a result, higher cell densities typically increase the risk of phage invasions. This has led to speculation that bacteria may have evolved strategies to counterbalance this increased susceptibility.

An MXene nanocomposite hydrogel for enhanced diabetic infected wound healing via photothermal antibacterial properties and bioactive molecule integration.

Diabetic wounds are a major clinical challenge due to their chronic, non-healing nature, which significantly impacts patients' quality of life. Traditional treatments often fail to effectively promote wound healing, highlighting the need for new biomaterials. In this study, we developed a composite hydrogel (KC@PF@TA) that combines the photothermal and antibacterial properties of Ti₃C₂Tx-Ag (Titanium carbide-silver) with the regenerative effects of paeoniflorin (PF).

Specific detection of Brucella spp. from slaughter-aged livestock using a dual priming oligonucleotide system.

Background: Brucella spp. are Gram-negative bacteria causing brucellosis, a major zoonotic disease affecting animals and humans. Annually, over 500,000 human cases are reported globally, with many undiagnosed due to nonspecific symptoms and diagnostic challenges.

Tumor cell-derived EMP1 is essential for cancer-associated fibroblast infiltration in tumor microenvironment of triple-negative breast cancer.

The role of epithelial membrane protein 1 (EMP1) in tumor microenvironment (TME) remodeling has not yet been elucidated. In addition, the biological function of EMP1 in triple-negative breast cancer (TNBC) is largely unclear. In this study, we examined the infiltration landscape of cell types in the TME of breast cancer, and found that EMP1 expression was positively correlated with stromal and microenvironmental scores.

New insights into microbial bile salt hydrolases: from physiological roles to potential applications.

Gut microbiota has been increasingly linked to metabolic health and diseases over the past few decades. Bile acids (BAs), the major components of bile, are bidirectionally linked to intestinal microbiota, also known as the gut microbiome-BA metabolic axis. Gut microbiota-derived bile salt hydrolase (BSH, EC 3.

DNA nanoframework-confined well-organized catalytic hairpin assembly for highly sensitive and specific detection of MiRNA.

MiRNAs (miRs) are emerging as valuable biomarker for disease monitoring, diagnosis, and prognostic evaluation, particularly cancer. Herein, we have developed a highly sensitive and specific fluorescent biosensor for miRNA detection using a DNA nanoframework-confined well-organized catalytic hairpin assembly (DOCO-CHA) system. The biosensor leverages DNA dendrimers, self-assembled from Y-DNA1 and Y-DNA2, to provide abundant intercalation sites for immobilizing a large number of catalytic hairpin assembly (CHA) probes, thereby enhancing the reaction environment and improving detection efficiency.

A YOLO-Based Model for Detecting Stored-Grain Insects on Surface of Grain Bulks.

Accurate, rapid, and intelligent stored-grain insect detection and counting are important for integrated pest management (IPM). Existing stored-grain insect pest detection models are often not suitable for detecting tiny insects on the surface of grain bulks and often require high computing resources and computational memory. Therefore, this study presents a YOLO-SGInsects model based on YOLOv8s for tiny stored-grain insect detection on the surface of grain bulk by adding a tiny object detection layer (TODL), adjusting the neck network with an asymptotic feature pyramid network (AFPN), and incorporating a hybrid attention transformer (HAT) module into the backbone network.

Relationship between glucose to lymphocyte ratio and the first peritonitis episode in patients treated with peritoneal dialysis.

This study aimed to investigate the potential relationship between baseline glucose to lymphocyte ratio (GLR) levels and the first episode of peritonitis in patients treated with peritoneal dialysis (PD). A total of 314 patients treated with PD were included and divided into three groups based on GLR tertiles: tertile 1 (GLR ≤ 4.23); tertile 2 (4.

Engineered cellulosic triboelectric materials for multi-modal sensing via β-phase programming.

Cellulosic triboelectric materials have gained widespread attention in triboelectric nanogenerators (TENGs) recently due to their fascinating merits of renewability, biodegradability, and low cost. However, cellulose-based TENGs still are challenged by the low surface charge density of triboelectric materials. Herein, the negative charged cellulosic triboelectric materials are functionalized as triboelectric layers for self-powered sensors via an electrostatic spinning method, where the electrospun polyvinylidene fluoride (PVDF) nanofibers fill with the porous air-laid paper (AP), boosting the formation of polar β-phase crystals and a negative shift of the surface potential.

Addition of anti-PD-1 immunotherapy to BRAF inhibitor-based targeted therapy improves real-world survival and delays brain metastases in patients with BRAF-mutant advanced melanoma: a multicenter cohort study.

Anti-PD-1 immunotherapy and targeted therapy (TT) represent two major therapeutic modalities for BRAF-mutant advanced melanoma, but the efficacy of combination therapy in Asian populations remains unknown. Asian melanoma patients differ significantly from Caucasians in tissue subtypes, pathogenesis and response to treatment. We retrospectively analyzed data of BRAF-mutant advanced melanoma patients treated with first-line vemurafenib (V) ± anti-PD-1 or dabrafenib+trametinib (D+T) ± anti-PD-1 between 2014 and 2023 from three centers in China.

Functional variant at 19q13.3 confers nonsyndromic cleft palate susceptibility by regulating .

This study investigates the genetic factors associated with nonsyndromic cleft palate only (NSCPO) through a two-stage genome-wide association study (GWAS) and functional experiments. A total of 311 NSCPO cases and 1,136 controls were included, and we identified rs3826795 in significantly associated with NSCPO risk ( = 4.27E-09).

Molecular investigation reveals three hemotropic mycoplasmas in cats and three tick species in China.

To date, the primary hemoplasmas that infect cats include , Mycoplasma haemominutum, and Mycoplasma turicensis. In addition, other hemoplasmas species have also been identified in cats. In central China, no infections or potential vectors with hemotropic mycoplasmas have been recorded in cats.

TREM2 protects against LPS-induced murine acute lung injury through suppressing macrophage ferroptosis.

Triggering receptor expressed on myeloid cells-2 (TREM2) is a cell surface receptor, majorly expressed by granulocytes, monocytes and macrophages. We in this study showed that TREM2 was downregulated in the lipopolysaccharide (LPS)-treated macrophages and murine acute lung injury (ALI) through activation of p38 MAPK and STAT6 signaling. Over-expression of TREM2 reduced the expression of DNAX-activation protein 12 (DAP12), pro-inflammatory cytokines (TNF-α, IL-6, IL-1β), Malondialdehyde (MDA) and hemosiderin accumulation in LPS-treated macrophages.

The cytological mechanism of the peach haploid producing triploid offspring.

Peach is one of the most economically valuable fruit trees. Haploid peach trees occur spontaneously at very low frequencies and they are usually highly sterile. Therefore, the haploid with partial fertility is an extremely rare germplasm, which is highly valuable to genetic research and breeding programs.

Multifaceted Antipathogenic Activity of Two Novel Natural Products, Chermesiterpenoid B and Chermesiterpenoid B Seco Acid Methyl Ester, Against Pseudomonas aeruginosa.

Pseudomonas aeruginosa is an opportunistic human pathogen that causes both acute and chronic infections due to its virulence factors, biofilm formation and the ability to suppress the host immune system. Quorum sensing (QS) plays a key role in regulating these pathogenic traits and also downregulates the expression of peroxisome proliferator-activated receptor-γ (PPAR-γ) in host cells. In this study, we isolated two novel natural products from the jellyfish-derived fungus Penicillium chermesinum, chermesiterpenoid B (Che B) seco acid methyl ester (Che B ester) and Che B.

A Self-Assembled Amino Acid Hydrogel for Immobilization and Protection of Enzymes.

Enzymes are essential biological catalysts, which have merits such as specificity, high efficiency, and mild-acting conditions. Due to the characteristics of enzymes, problems such as poor operational stability and difficulty in reuse limit the practical application of enzymes. These problems can often be solved by immobilization of enzymes.

Seedling size affects the strategy of dune seedlings responding to rainfall variation.

Background And Aims: Climate change poses a growing threat to population maintenance in harsh desert habitats with high precipitation variability. Desert seeds may germinate at different times as a bet-hedging strategy to cope with increased rainfall fluctuation. As a result, a population may consist of seedlings of various sizes.

Perioperative tislelizumab plus chemotherapy for locally advanced gastroesophageal junction adenocarcinoma (NEOSUMMIT-03): a prospective, nonrandomized, open-label, phase 2 trial.

This prospective, nonrandomized, open-label phase 2 trial (Chinese Clinical Trial Registry, ChiCTR2200061906) aimed to evaluate the effectiveness of adding the PD-1 antibody tislelizumab to perioperative chemotherapy in patients with locally advanced gastroesophageal junction adenocarcinoma (GEJA). This study enrolled patients with GEJA clinically staged as cT3-4aNanyM0 or cT1-2N+M0 from October 2022 to June 2023. Eligible patients were administered three preoperative and five postoperative 3-week cycles of treatment with PD-1 antibody tislelizumab plus SOX (S-1 and oxaliplatin) regimen.