[Standarization of the plaque-reduction neutralization technique in Vero cells for dengue virus testing].

Introduction: the standard plaque reduction neutralization technique has been performed in BHK-21 cells for more than 20 years in Cuba to determine the neutralizing antibodies to dengue. At the end of 2007, the WHO implemented a program to harmonize this technique at all the laboratories worldwide.

Objectives: the present study was aimed at standardizing the plaque-reduction neutralization technique in Vero cells from the Cultural Lab of"Pedro Kouri" Tropical Medicine Institute.