Amino Acid Analog Induces Stress Response in Marine .

Characterizing the cell-level metabolic trade-offs that phytoplankton exhibit in response to changing environmental conditions is important for predicting the impact of these changes on marine food web dynamics and biogeochemical cycling. The time-selective proteome-labeling approach, bioorthogonal noncanonical amino acid tagging (BONCAT), has potential to provide insight into differential allocation of resources at the cellular level, especially when coupled with proteomics. However, the application of this technique in marine phytoplankton remains limited.

Diet breadth and exploitation of exotic plants shift the core microbiome of , a group of tropical herbivorous beetles.

The beetle genus has evolved in association with tropical ginger plants and for many species their specific host plant associations are known. Here we show that the core microbiome of six closely related Costa Rican species comprises only eight bacterial groups, including members of the , Enterobacteriacea, , and . The and Enterobacteriacea together accounted for 35% of the total average 16S rRNA ribotypes recovered from all specimens.