[Comparison of Malaria Diagnosis between County and Provincial Laboratories in Guizhou Province].

Malaria cases reported by county laboratories were further tested in the provincial laboratory in Guizhou province by using PCR test and microscopy. The consistency between PCR and microscopic results in the provincial laboratory was set as the basis for evaluation of microscopic results in county laboratories. In 89 samples, 24 were identified by PCR to be positive for malaria, among which 15 were infected with P.

[Single-tube Single-run Multiplex PCR Detects Mixed Samples with Four Species of Plasmodium].

Objective: To establish a single-tube single-run multiplex PCR technique that can detect single or mixed samples with four species of Plasmodium.

Methods: Folding primers were designed based on the fast nested PCR. The reaction component concentrations were optimized and the primers were selected based on the annealing temperature.