[Peptide bond scission of staphylococcal enterotoxin C2 and related factors].

Objective: To investigate the limited digestion of recombinant staphylococcal enterotoxin C2 (SEC2-His)in different conditions.

Methods: The purified recombinant SEC2-His was treated with different reagents and the cleavage of rSEC2 molecule was observed by SDS-PAGE.

Result: The cleavage occurred in positions Cys93-Cys110 of the disulfide loop.

Expression and bioactivity analysis of Staphylococcal enterotoxin M and N.

Staphylococcal enterotoxins (SEs) are powerful superantigens that stimulate non-specific T-cell proliferation produced by Staphylococcus aureus and draw considerable attention as ideal drugs for cancer therapy. The filtrate of S. aureus culture has been used as ampul named Staphylococcal enterotoxin C injection in clinic for 10 years in China and proved to be effective.

[Expression and bioactivity analysis of staphylococcal enterotoxin C2].

Aim: To clone the gene of staphylococcal enterotoxin C2 and express it in the form of a soluble fusion protein in E. coli. Then the activation of SEC2 on mice lymphocyte and its lethal effects on tumor cells were studied.