Quantitative analyses of CD7, CD33, CD34, CD56, and CD123 within the -ITD/-MUT myeloblastic/monocytic bulk AML blastic populations.

The most frequent mutations in acute myeloid leukemia (AML) - -ITD and - are associated with a specific immunophenotype. We evaluated the levels of surface antigens in an uninvestigated AML patient population according to the combination of -ITD/ mutations. Antigen levels were calculated as the geometric mean fluorescence index (MFI) ratio between myeloblasts or monoblasts/monocytes and a negative population for the specific antigen.

Evaluation of Commercial qPCR Kits for Detection of SARS-CoV-2 in Pooled Samples.

Due to the current pandemic, a global shortage of reagents has drawn interest in developing alternatives to increase the number of coronavirus tests. One such alternative is sample pooling. We compared commercial kits that are used in COVID-19 diagnostics in terms of their sensitivity and feasibility for use in pooling.

FLT3-ITD DNA and mRNA levels in AML do not correlate with CD7, CD33 and CD123 expression.

Introduction: FLT3 internal tandem duplication (ITD) mutations are found in around 25% of all acute myeloid leukaemia (AML) cases and is associated with shorter disease-free and overall survival. Previous reports have shown that FLT3-ITD induces a specific phenotype in leukemic blasts, which is characterized by high levels of CD33 and CD123, and that expression of CD33 and CD123 is directly influenced by the DNA FLT3-ITD/wild-type FLT3 allelic ratio (AR).

Methods: A total of 42 FLT3-ITD and 104 FLT3-ITD-negative AML patients were analysed.