Transcriptome profiling of foxtail millet (Setaria italica) pollen and anther.

Pollen development and germination play a crucial role in the sexual reproduction of plants. This study analysis of transcriptional dynamics of foxtail millet pollen with other tissues and organs (ovule, glume, seedling and root) through RNA-sequencing revealed that a total of 940 genes were up-regulated in foxtail millet pollen. Based on this, we analyzed the genes involved in pollen tube growth of receptor kinases and small peptides, calcium signaling, small G proteins, vesicle transport, cytoskeleton, cell wall correlation, and transcription factors that are up-regulated in pollen.

Optimal timing of anti-PD-1 antibody combined with chemotherapy administration in patients with NSCLC.

Background: Anti-programmed cell death 1 (PD-1) antibody combined with chemotherapy simultaneously is regarded as the standard treatment for patients with advanced non-small cell lung cancer (NSCLC) by current clinical guidelines. Different immune statuses induced by chemotherapy considerably affect the synergistic effects of the chemo-anti-PD-1 combination. Therefore, it is necessary to determine the optimal timing of combination treatment administration.

Glucose Abnormalities Detected by Continuous Glucose Monitoring in Very Old Adults With and Without Diabetes.

Objective: To characterize the prevalence of continuous glucose monitoring (CGM)-defined glucose abnormalities in a large, community-based population of very old adults (>75 years).

Research Design And Methods: A cross-sectional analysis of 1,150 older adults with and without diabetes who attended the Atherosclerosis Risk in Communities Study (2021-2022). Diabetes was based on a self-reported diagnosis of diabetes by a health care provider, use of diabetes medication, or a hemoglobin A1c (HbA1c) ≥6.

The priority state of items in visual working memory determines their influence on early visual processing.

Items held in visual working memory (VWM) influence early visual processing by enhancing memory-matching visual input. Depending on current task demands, memory items can have different priority states. Here, we investigated how the priority state of items in VWM affects two key aspects of early visual processing: access to visual awareness and attention allocation.

Aptamer-Based Activatable Tyramide Signal Amplification for Low-Background Detection of SARS-CoV-2 Nucleocapsid Protein.

As severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection posed a significant threat to public health and the global economy, in vitro diagnosis of the SARS-CoV-2 nucleocapsid protein proved to be an effective way for SARS-CoV-2 infection control in the past years. Tyramide signal amplification (TSA) has been extensively utilized in tissue imaging and pathological diagnosis owing to the powerful signal enhancement. However, the elevated "ALWAYS ON" fluorescence background limited the accuracy and sensitivity of the conventional TSA assay.

Influencing factors of ocular pain in dry eye disease patients at high altitude.

Aim: To quantify the severity and frequency of ocular pain in Tibetan plateau patients with dry eye, and to evaluate the related factors affecting ocular pain.

Methods: A retrospective study included 160 cases of dry eye disease (DED) patients who were treated from July 2022 to June 2023. Age, gender, occupation, illness course, anxiety, plateau duration, plateau protection, ocular surface disease index scale (OSDI), break-up time (BUT), Schirmer I test (SIT), conjunctivitis, history of ophthalmic medication, autoimmune disease, the workload of daily near vision range, smoking and overnight stay were obtained comprehensive ophthalmic assessment, and their duration was followed up.

Variable Valence Ce-Based CsCeAgBr Perovskite Nanocrystals for Highly Selective Photoconversion of CO to CH.

Lead halide perovskites demonstrate outstanding luminescent characteristics. However, the inclusion of lead components restricts their extensive utilization. Halide perovskite materials, formulated as AM(III)M(I)X or AM(IV)X, possess the potential to serve as stable and eco-friendly substitutes for optoelectronic applications.

Evaluating pregnancy outcomes in women with uterine fibroids treated with high-intensity focused ultrasound: insights from a single-institution study.

Objective: This study aims to assess the safety and effectiveness of High-Intensity Focused Ultrasound (HIFU) ablation on pregnancy outcomes among women with uterine fibroids wishing to conceive, focusing specifically on short-term pregnancy outcomes immediately following HIFU treatment.

Methods: A retrospective analysis was conducted on 210 women who underwent HIFU treatment (Group I) at our institution between January 2018 and December 2022 and subsequently conceived. Pregnancy outcomes were compared with two control groups: 510 patients who delivered vaginally (Group II) and 278 who underwent cesarean sections (Group III) during the same period.

Serum metabolic alterations in chickens upon infectious bursal disease virus infection.

Background: Infectious bursal disease virus (IBDV) is a highly contagious immunosuppressive virus of chickens. Chickens acquire infection by the oral route under natural conditions. Although the histological and pathological changes after IBDV infection are well described, the alterations in serum metabolome have not been reported.

The 24-kDa subunit of mitochondrial complex I regulates growth, microsclerotia development, stress tolerance, and virulence in Verticillium dahliae.

Background: The complete mitochondrial respiratory chain is a precondition for maintaining cellular energy supply, development, and metabolic balance. Due to the evolutionary differentiation of complexes and the semi-autonomy of mitochondria, respiratory chain subunits have become critical targets for crop improvement and fungal control. In fungi, mitochondrial complex I mediates growth and metabolism.

Suppressed P3-O3' Phase Transition and Enhanced Na Diffusion Kinetics of O3-Type Layered Oxide Cathode via Multivariate Doping.

O3-NaNiFeMnO has attracted much attention as a cathode for sodium-ion batteries, because of its low cost and high sodium-ion storage capacity. However, its slow Na diffusion kinetics and harmful P3-O3' phase transition with severe bulk strain at high voltage leads to poor rate capability and fast capacity fading. Herein, we propose a multivariate doping strategy with Cu, Mg, and Ti ions to solve the above problems of the O3-NaNiFeMnO cathode.

Optimization of the extraction process of goji berry pectin using response surface methodology and its suitability as thickener for yogurt.

Although goji berry with many main biological activity components is a nutritional medicinal and edible plan and widespread in the northeast of China, its current development also faces a new challenge of overcapacity. So this study was largely focused to investigate the optimal extraction process conditions (extraction water proportion, acidic pH regulating reagent, adjusted pH, extraction temperature, extraction time) for goji berry with increased yield. Box Behnken experimental design (BBD) was used to model the mathematical model of the relationship between the three main influence of pivotal extraction parameters (extraction pH, extraction time and extraction temperature) selected by the single factor experiments on the responses of goji berry pectin yield (%).

Analysis of the effect of LMO2 on indicating CD8 T-lymphocyte infiltration in pan-cancers.

LMO2 is a critical factor in hematopoiesis but widely expressed in kinds of epithelia and solid tumors as well, and it has been demonstrated that increased LMO2 level in some tumor cells exhibits an effect on promoting CD8 T-lymphocyte infiltration in tumor microenvironment. Herein we expanded the investigation of such effect in pan-cancer spectrum, and the transcriptome data analysis revealed extensively positive associations between LMO2 and CD8 T-lymphocyte infiltration in pan-cancers. Meanwhile, tissue staining revealed variable expression level and consistently cytosolic localization of LMO2 in different tissues and their tumor counterparts, and further indicated that high LMO2 expression in malignant cells was strongly associated with positive CD8 T-lymphocyte infiltration selectively in digestive tract, breast and kidney tumors.

Transcriptome Profiling Reveals Key Regulatory Networks for Age-Dependent Vernalization in Welsh Onion ( L.).

Plants exhibit diverse pathways to regulate the timing of flowering. Some plant species require a vegetative phase before being able to perceive cold stimuli for the acceleration of flowering through vernalization. This research confirms the correlation between the vernalization process and seedling age in Welsh onions.

Improved Antitumor Efficiency of -Tetradecyloxycarbonyl Gemcitabine-Loaded Liposomes for Pancreatic Cancer Chemotherapy.

Background: Gemcitabine (Gem) is one of the first-line chemotherapy drugs for pancreatic cancer treatment. However, its short half-life in plasma and adverse effects limited its broader application.

Methods: A novel Gem derivative ( -tetradecyloxycarbonyl gemcitabine, tcGem) was synthesized and encapsulated into liposomes (LipotcGem) to overcome the above shortcomings.

Histamine H2 Receptor Antagonists in the Treatment and Prevention of Heart Failure.

Despite advancements in the treatment of heart failure (HF) and modest improvements in survival rates over the past few decades, mortality rate remains significantly high. HF not only imposes a significant economic burden on patients' families but also presents a substantial challenge to society at large. Therefore, effective treatment and prevention strategies are crucial.

AURKA inhibits the decidualization of the eutopic endometrium in endometriosis through NF-κB p65†.

Endometriosis is an estrogen dependent disease, which is related to infertility. Decidualization is a prerequisite for successful implantation of human embryos, and endometriosis affects the occurrence of decidualization. However, the mechanism that affects decidualization in endometriosis is not fully understood.

The Role of Wnt5a in Inflammatory Diseases.

Wnt5a plays an important role in cell development and maturation and is closely associated with various diseases, such as malignant tumours, metabolic disorders, fibrosis, growth and development. Recent studies have shown that Wnt5a expression and signal transduction are strongly involved in the inflammatory response. This study comprehensively reviewed the latest research progress on the association between Wnt5a and several inflammatory diseases, such as sepsis, asthma, chronic obstructive pulmonary disease, tuberculosis, rheumatoid arthritis, atherosclerosis and psoriasis vulgare.

Clinical value of esketamine combined with ropivacaine in rebound pain after brachial plexus block in patients with upper limb fractures.

Objective: To analyze the clinical value of the combination of esketamine and ropivacaine in alleviating rebound pain in patients with upper limb fractures following brachial plexus block.

Methods: A total of 149 patients with unilateral upper limb fractures who underwent open reduction and internal fixation surgery under brachial plexus block anesthesia from November 2021 to August 2022 were selected as the subjects for the study and randomly divided into the esketamine group (RNK group) and the ropivacaine group (R group). The incidence of rebound pain at 48 h postoperatively, intraoperative mean arterial pressure (MAP) and heart rate (HR), the onset time and duration of blockade, the Numeric Rating Scale (NRS) scores for pain at rest and with activity during the perioperative period, the dosage, numbers of compressions, and effective compressions of postoperative patient-controlled analgesia with sufentanil, and occurrence of adverse reactions were assessed and compared between the two groups.

A Case of Acute Necrotic Encephalopathy Associated with Influenza A Virus in Adults.

Background: Acute necrotizing encephalopathy is a rare acute, explosive, and severe form of encephalopathy that predominantly occurs in children; however, it is infrequent in adults. The patient is typically caused by viral infection, with rapid onset of fever, convulsion, disturbance of consciousness, and other symptoms. It presents symmetrical, multifocal, involving bilateral thalamic damage and other typical imaging features.

Prognostic indicators and outcome in patients with acute liver failure, sepsis and with and without shock: a retrospective cohort study.

Background: Sepsis or septic shock is associated with severe morbidity and mortality in patients with acute liver failure (ALF). This study aimed to explore the potential prognostic value of common clinical indicators in patients with ALF, sepsis and with and without shock.

Patients And Methods: The clinical, laboratory, and microbiological data of patients with ALF and sepsis or septic shock who were admitted to the intensive care unit from January 2014 to December 2019 were collected retrospectively.

Corrigendum: SERS-based microdroplet platform for high-throughput screening of strains for the efficient biosynthesis of D-phenyllactic acid.

[This corrects the article DOI: 10.3389/fbioe.2024.

Analyzing the potential targets and mechanisms of chronic kidney disease induced by common synthetic Endocrine Disrupting Compounds (EDCs) in Chinese surface water environment using network toxicology and molecular docking techniques.

Long-term exposure to NP and OP, as common synthetic endocrine-disrupting chemicals (EDCs) in surface water environments in China, is closely associated with the development of chronic kidney disease (CKD). However, their potential targets and toxicological mechanisms for inducing CKD remain unknown. This study utilizes network toxicology and molecular docking techniques to explore the potential toxic targets and molecular mechanisms of CKD induction by NP and OP.

Allergen-specific sublingual immunotherapy altered gut microbiota in patients with allergic rhinitis.

Introduction: Allergen-specific immunotherapy (AIT) induces long-term immune tolerance to allergens and is effective for treating allergic rhinitis (AR). However, the impact of sublingual immunotherapy (SLIT) on gut microbiota from AR patients and its correlation with treatment efficacy remains unclear.

Methods: In the present study, we enrolled 24 AR patients sensitized to Dermatophagoides farinae (Der-f) and 6 healthy donors (HD).

[ (Blume) Miq] maintains uric acid homeostasis via regulating gut microbiota and restrains renal inflammation in hyperuricemic nephropathy.

Introduction: The kidney damage caused by the deposition of uric acid in the kidneys is of urgent need for new treatment drugs due to its complex pathogenesis. (Blume) Miq. Also known as , which has a significant therapeutic effect on hyperuricemia nephropathy (HN), however, the specific mechanism of its action is still unknown.