[The Thyroid Hormone Receptor Mediated Luciferase Reporter Gene Assays Screening for EDCs].

Objective: This study in order to use report gene assay based on the thyroid hormone receptor (TR) α/β from human origin for screening endocrine disruptors chemicals (EDCs), evaluating the thyroid hormone activity of Bisphenol (BPA), 1-Naphthaleny methyl carbamate and 1-naphthol (1-NAP).

Methods: Using Rhesus monkey kidney cells (LLC-MK2) as transfection cell to establish the gene report assay system based on pGL-3-promega and pGL4.27 of TRα/β through the method of transient transfection.