Evaluation of delivery agents used for introduction of small interfering RNAs into feline corneal cells.

Objective: To evaluate agents used for delivery of small interfering RNAs (siRNAs) into feline corneal cells, toxicity of the delivery agents, and functionality of anti-feline herpesvirus 1 (FHV-1)-specific siRNA combinations.

Sample: Feline primary corneal cells and 19 six-month-old colony-bred cats.

Procedures: siRNA delivery into corneal cells via various delivery agents was evaluated via flow cytometric detection of labeled siRNAs.

Effects of oral administration of methazolamide on intraocular pressure and aqueous humor flow rate in clinically normal dogs.

Objective: To determine magnitude and duration of the effect of oral administration of methazolamide at 2 dosages on intraocular pressure (IOP) in dogs in single-dose and multiple-dose trials and to determine aqueous humor flow rate (AHFR) by use of anterior segment fluorophotometry before and during treatment.

Animals: 25 healthy adult Beagles.

Procedure: Baseline IOPs and AHFRs were determined on days 0 and 1, respectively.