Wu Mei Wan suppresses colorectal cancer stemness by regulating Sox9 expression via JAK2/STAT3 pathway.

Ethnopharmacological Relevance: Wu Mei Wan (WMW) is a traditional Chinese herbal formula with a long-standing history in Chinese medicine, valued for its therapeutic properties. However, its potential anti-cancer effects, especially against colorectal cancer (CRC), have not been fully elucidated.

Aim Of The Study: This study aims to investigate the effects of WMW on colorectal cancer stemness and to elucidate the underlying molecular mechanisms, focusing on the modulation of Sox9 expression via the JAK2/STAT3 signaling pathway.

[Effects of electroacupuncture on the inflammatory response and intestinal flora in obese rats].

Objectives: To observe the effect of electroacupuncture (EA) on the expressions of high mobility group protein 1(HMGB1) and myeloid differentiation factor 88 (MyD88) in the small intestine and intestinal flora of obese rats, so as to explore the potential mechanism of EA to improve obesity in rats.

Methods: After 1 week of acclimatization, 10 rats were randomly selected from 50 Wistar male rats as the normal group, and the rest rats were fed with high-fat diet for 8 weeks to establish the obese model. The successfully modeling rats were randomly divided into model group, EA group and sham EA group, with 10 rats in each group.

The mechanism of ITGB4 in tumor migration and invasion.

Integrin β4 (ITGB4) is a transmembrane protein that functions as a mechanosensor, mediating the bidirectional exchange of information between the intracellular and extracellular matrices. ITGB4 plays a critical role in cell adhesion, migration, and signaling. Numerous studies have implicated ITGB4 as a key facilitator of tumor migration and invasion.

Investigation on Plastic Flow Behaviors of FCC Polycrystalline Aluminum under Pre-Cyclic Tension-Compression Loading: Experiments and Crystal Plasticity Modeling.

The plastic flow behaviors of FCC polycrystalline aluminum after pre-cyclic tension-compression deformation are mainly investigated in tension-torsion stress space by the physically based crystal plasticity model introducing a back-stress. A global finite element model (GFEM) constructed of sufficient grains was established to simulate the same-size thin-walled tube specimen constrained and loaded as the experiments of yield surfaces. The computational results showed that the shape of subsequent yield surfaces and the plastic flow directions directly depended on the given offset strain levels and the applied re-loading paths under different pre-cyclic deformations.

Hydrogen-Assisted Brittle Fracture Behavior of Low Alloy 30CrMo Steel Based on the Combination of Experimental and Numerical Analyses.

Compact-tension (CT) specimens made of low alloy 30CrMo steels were hydrogen-charged, and then subjected to the fracture toughness test. The experimental results revealed that the higher crack propagation and the lower crack growth resistance (CTOD-R curve) are significantly noticeable with increasing hydrogen embrittlement (HE) indexes. Moreover, the transition in the microstructural fracture mechanism from ductile (microvoid coalescence (MVC)) without hydrogen to a mixed quasi-cleavage (QC) fracture and QC + intergranular (IG) fracture with hydrogen was observed.

Torsional Fatigue Life Prediction of 30CrMnSiNi2A Based on Meso-Inhomogeneous Deformation.

In this paper, torsional fatigue failure of 30CrMnSiNi2A steel which exhibited non-Masing behavior was studied under different constant shear strain amplitudes, using thin-walled tubular specimens. The relationship between shear fatigue and the evolution of meso-deformation inhomogeneity and the prediction method of the torsional fatigue life curve were investigated. Shear fatigue of the material under constant amplitude was researched by numerical simulation with reference to tests, by using crystal plasticity of polycrystalline representative volume element (RVE) as the material model.

Investigation of Yield Surfaces Evolution for Polycrystalline Aluminum after Pre-Cyclic Loading by Experiment and Crystal Plasticity Simulation.

This study aims at introducing the back stress of anisotropic strain-hardening into the crystal plasticity theory and demonstrating the rationality of this crystal plasticity model to describe the evolution of the subsequent yield surface of polycrystalline aluminum at the mesoscopic scale under complex pre-cyclic loading paths. By using two different scale finite element models, namely a global finite element model (GFEM) as the same size of the thin-walled tube specimen used in the experiments and a 3D cubic polycrystalline aggregate representative volume element (RVE) model, the evolution of the subsequent yield surface for different unloading cases after 30 pre-cycles is further performed by experiments and numerical simulations within a crystal plasticity finite element (CPFE) frame. Results show that the size and shape of the subsequent yield surfaces are extremely sensitive to the chosen offset strain and the pre-cyclic loading direction, which present pronounced anisotropic hardening through a translation and a distortion of the yield surface characterized by the obvious "sharp corner" in the pre-deformation direction and "flat" in the reverse direction by the definition of small offset strain, while the subsequent yield surface exhibits isotropic hardening reflected by the von Mises circle to be distorted into an ellipse by the definition of large offset strain.

Statistical Analysis of Grain-Scale Effects of Twinning Deformation for Magnesium Alloys under Cyclic Strain Loading.

To reveal the relationship between grain size and twinning deformation of magnesium alloys under cyclic strain, this study carried out a group of strain-controlled low-cycle fatigue experiments and statistical analysis of microstructures. Experimental results show that the shape of the hysteresis loop exhibits significant asymmetry at different strain amplitudes, and the accumulation of residual twins plays an important role in subsequent cyclic deformation. For the different strain amplitudes, the statistical distribution of the grain size of magnesium alloy approximately follows the Weibull probability function distribution, while the statistical distribution of twin thickness is closer to that of Gaussian probability function.

Prediction of Strain Fatigue Life of HRB400 Steel Based on Meso-Deformation Inhomogeneity.

The relationship between strain fatigue life and evolution of meso-deformation inhomogeneity was studied, through the cyclic process of numerical simulation of crystal plasticity compared with the fatigue test of steel hot-rolled ribbed-steel bar 400 (HRB400). The statistical characterization parameters at grain level, including the standard deviation of the dot product of longitudinal stress and strain, the product of the macro stress and the standard deviation of the longitudinal strain, and the product of the macro stress ratio and the standard deviation of the longitudinal strain, were proposed and respectively applied to measure the meso-deformation inhomogeneity of materials. These parameters take the effect of peak stress into account, distinct from the pure strain statistical parameters.