Publications by authors named "Damien Mouvet"
Article Synopsis
- Therapeutic oligonucleotides are increasingly important in medicine, but their manufacturing can lead to impurities like truncated species that affect quality and safety.
- A newly optimized weak anion exchange chromatography method effectively separates these impurities by improving the mobile phase's complexity and stability.
- A robustness study evaluated potential risks to ensure the reliability of this method, establishing a robust space for consistent purity determination of oligonucleotides.
A new analytical method based on ICP-MS/MS is proposed for the characterization of synthetic phosphorothioate oligonucleotides. Absolute quantification of oligonucleotides is challenging, as well as the determination of phosphodiester to phosphorothioate ratio for phosphorothioate oligonucleotides. Both are considered as critical quality attributes and should be determined using robust validated methods.
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