New erythrocyte parameters derived from the Coulter principle relate with red blood cell properties-A pilot study in diabetes mellitus.

In routine hematological instruments, blood cells are counted and sized by monitoring the impedance signals induced during their passage through a Coulter orifice. However, only signals associated with centered paths in the aperture are considered for analysis, while the rejected measurements, caused by near-wall trajectories, can provide additional information on red blood cells (RBC), as recent publications suggest. To assess usefulness of two new parameters in describing alterations in RBC properties, we performed a pilot study to compare blood samples from patients with diabetes mellitus (DM), frequent pathological condition associated with impairment in RBC deformability, versus controls.

Red blood cell rheology during a complete blood count: A proof of concept.

Counting and sizing blood cells in hematological analyzers is achieved using the Coulter principle. The cells flow in a micro-aperture in which a strong electrical field is imposed, so that an electrical perturbation, called pulse, is measured each time a cell crosses the orifice. The pulses are expected to contain information on the shape and deformability of Red Blood Cells (RBCs), since recent studies state that RBCs rotate and deform in the micro-orifice.

Detecting cells rotations for increasing the robustness of cell sizing by impedance measurements, with or without machine learning.

The Coulter principle is a widespread technique for sizing red blood cells (RBCs) in hematological analyzers. It is based on the monitoring of the electrical perturbations generated by cells passing through a micro-orifice, in which a concentrated electrical field is imposed by two electrodes. However, artifacts associated with near-wall passages in the sensing region are known to skew the statistics for RBCs sizing.

Detection of circulating microfilariae in canine EDTA blood using lens-free technology: preliminary results.

causes life-threatening heart disease in dogs, thus screening of dog populations is important. Lens-free technology (LFT) is a low-cost imaging technique based on light diffraction that allows computerized recognition of small objects in holographic images. We evaluated an algorithm capable of recognizing microfilariae in canine whole blood using the LFT.

Numerical simulation of deformable particles in a Coulter counter.

In Coulter counters, cells counting and volumetry are achieved by monitoring their electrical print when they flow through a sensing zone. However, the volume measurement may be impaired by the cell dynamics, which may be difficult to control. In this paper, numerical simulations of the dynamics and electrical signature of red blood cells in a Coulter counter are presented, accounting for the deformability of the cells.

Numerical simulation of particle dynamics in an orifice-electrode system. Application to counting and sizing by impedance measurement.

This paper describes how to numerically tackle the problem of counting and sizing particles by impedance measurement in an orifice-electrode system. The model allows to simulate the particle dynamics submitted to strong hydrodynamic stresses through a microorifice and to compute the voltage pulses generated by the modification of the inner dielectric medium. This approach gives important information about particles size distribution and allows to quantify the role of trajectory and orientation of particles on the size measurement.