- Conventional methods for identifying emerging pathogens, like selective culture assays and serological tests, have limitations including misidentifications and dependence on specific conditions that can hinder accurate diagnosis.
- Polymerase-chain-reaction (PCR) has improved diagnostic capabilities, but relies on published genetic sequences and knowledge of genetic diversity, which can complicate testing for environmental samples mixed with other DNA.
- Focusing on Buruli ulcer, this text argues that various mycolactone-producing mycobacteria should be viewed as variants of the same group instead of separate species, highlighting the importance of accurate molecular markers for understanding the disease's dynamics and emergence.