Publications by authors named "Dalton R Brown"
Article Synopsis
- - The authors found that co-crystallizing fluorophores with MALDI imaging matrices can boost fluorophore brightness significantly (up to 79 times), enhancing tissue autofluorescence.
- - This method, called FluoMALDI, allows simultaneous imaging of biological samples using both fluorescence microscopy and MALDI imaging, simplifying the process since both methods can target the exact same cells without physical alterations.
- - The study demonstrates FluoMALDI's potential with various fluorophores in brain and kidney tissues, indicating it could advance imaging techniques in fields like cell biology and pathology.
Multimodal tissue imaging techniques that integrate two complementary modalities are powerful discovery tools for unraveling biological processes and identifying biomarkers of disease. Combining Raman spectroscopic imaging (RSI) and matrix-assisted laser-desorption/ionization (MALDI) mass spectrometry imaging (MSI) to obtain fused images with the advantages of both modalities has the potential of providing spatially resolved, sensitive, specific biomolecular information, but has so far involved two separate sample preparations, or even consecutive tissue sections for RSI and MALDI MSI, resulting in images with inherent disparities. We have developed RaMALDI, a streamlined, integrated, multimodal imaging workflow of RSI and MALDI MSI, performed on a single tissue section with one sample preparation protocol.
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