Determination of 24 minor red blood cell antigens for more than 2000 blood donors by high-throughput DNA analysis.

Background: A "BeadChip" array permits reliable simultaneous DNA typing of single-nucleotide polymorphisms for minor blood groups. A high-throughput DNA analysis was studied as a routine method of phenotype prediction and software was developed to interpret and analyze the large volume of data points.

Study Design And Methods: DNA was extracted from whole blood of donors of known phenotypes and self-identified ethnicity.

A flexible array format for large-scale, rapid blood group DNA typing.

Background: Typing for blood group antigens is currently performed by hemagglutination. The necessary reagents are becoming costly and limited in availability, and the methods are labor-intensive. The purpose of this study was to determine the feasibility of the use of large-scale DNA analysis in a microarray as a substitute for blood group typing.