Euonymus europaeus lectin as an endothelial and epithelial marker in canine tissues.

The Euonymus europaeus agglutinin (EEA) is an endothelial marker in mammalia. In canine tissues, 4 types of endothelial cells (general, nervous, arterial, hepatic) were identified by the presence of the EEA receptor and by its sensitivity to neuraminidase enhancement. In adult dogs, EEA binding saccharides had endothelial or epithelial distributions and reactivities similar to those described for human tissues.

Lectins as markers of endothelial cells: comparative study between human and animal cells.

Vascular endothelial cells were labelled with 10 vegetal lectins and 3 more monoclonal antibodies antiblood group ABO substances, in major organs of 14 common laboratory animals. After fixation in PLPa and paraffin embedding, cells were examined to determine their likeness to human cells. The most interesting reactive used was EEA, whose positivity defines upper mammalians.

[Technical parameters influencing the reactivity of the vascular endothelium in relation to lectin of Evonymus europaeus].

Some parameters characterizing sample preparation for coloration with lectins were investigated in the model of sheep myocardial capillaries, revealed by EEA after formol fixation and paraffin embedding. The most influential factors are: addition of lysine-periodate to paraformaldehyde, duration of fixative immersion, oldness of paraformaldehyde dilution, delay of fixation (if below 8 h). All of them happen before embedding.

[Acute experimental pyelonephritis. Treatment with tobramycin. Influence of the rhythm of administration on efficacy and renal tolerability].

Acute experimental pyelonephritis was produced in rabbits by injecting E. coli (tobramycin MIC 1 mg/l) into the left kidney and temporarily obstructing the ureter. Animals were given 10 mg/kg tobramycin intramuscularly 48 h after surgery and subsequently every day for 7, 10 or 15 days, either in a single daily dose or in three divided doses at 8 h intervals.