Publications by authors named "Daisuke Tomura"
Article Synopsis
- TCR-engineered T cells targeting intracellular antigens like NY-ESO-1 show promise for treating solid tumors, but endogenous TCRs may hinder their effectiveness and cause self-reactivity.
- A phase I clinical trial tested a novel TCR-transduced T-cell product (TBI-1301) in patients with NY-ESO-1-expressing tumors, using siRNA to silence endogenous TCRs, with patients split into two dosing cohorts.
- Results indicated that while some patients exhibited tumor responses, early-onset cytokine release syndrome (CRS) occurred in several, and one experienced serious lung injury, highlighting the potential benefits and risks of TCR-T cell therapy.
Wilms' tumor 1 (WT1) is constantly expressed in leukemic cells of acute leukemia and myelodysplastic syndrome (MDS). A T-cell receptor (TCR) that specifically reacts with WT1 peptide in the context of HLA-A*24:02 has been identified. We conducted a first-in-human trial of TCR-gene transduced T-cell (TCR-T-cell) transfer in patients with refractory acute myeloblastic leukemia (AML) and high-risk MDS to investigate the safety and cell kinetics of the T cells.
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- Preparative lymphodepletion, which temporarily reduces the immune system, may help enhance the effectiveness of T-cell therapy, but its necessity for TCR-engineered T-cell therapy is still uncertain.
- A clinical trial was conducted with 10 patients suffering from recurrent esophageal cancer who received TCR gene-engineered T-cell transfers without any lymphocyte-depleting treatments or IL2 administration.
- The results revealed that while some TCR-transduced cells persisted in the patients for extended periods, most showed tumor progression, indicating that T-cell persistence doesn’t always correlate with tumor regression and that other factors might influence treatment outcomes.
Cloning and Expression of the Cytochrome P-450nor2 cDNA from Cylindrocarpon tonkinense.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai)
January 1996
Cytochrome P-450nor is involved in the fungal denitrification and acts as a nitric oxide reductase. The cDNA library from Cylindrocarpon tonkinense was constructed with lambdagtll, and screened with antibodies. From the positive clones, the P-450nor2 cDNA fragments were recovered, and subcloned into the expression vector pYES2, then expressed in the yeast system.
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