Publications by authors named "Daisuke Tanaka"

Article Synopsis
  • * The researchers developed a new method called TRISCO, which helps retain RNA signals in cleared organs for better imaging, specifically for whole-brain 3D RNA visualization.
  • * TRISCO effectively preserves RNA integrity, ensures uniform labeling, and enhances tissue transparency, making it a powerful tool for detailed transcriptional spatial analysis at the single-cell level across various organs.
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  • Biologically precipitated magnetite (FeO) nanocrystals are found in various organisms, including plants, and they can act as ice nucleation particles (INPs) that initiate freezing in supercooled water.
  • Research on garlic cloves showed that these magnetite INPs might trigger frost damage; when subjected to different magnetic fields, the temperature distribution of supercooling was significantly affected.
  • The study found that while weak magnetic fields increased freezing chances, stronger fields decreased it, suggesting the presence of loose binding agents on the magnetite that influence ice nucleation.
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  • The Japan Diabetes Society (JDS) has formed a committee to regularly update consensus statements on diabetes management, starting with guidelines on "Medical Nutrition Therapy" in 2020, and a pharmacotherapy algorithm for type 2 diabetes in 2022.
  • The pharmacotherapy algorithm emphasizes tailoring medication choices to individual patients' diabetes pathology while also considering clinical evidence and prescribing practices in Japan.
  • Recent revisions to these consensus statements include the addition of tirzepatide—approved in April 2023—as a treatment option, alongside detailed assessments for insulin resistance based on new criteria for management.
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  • - This algorithm outlines guidelines for the safe and effective use of medications to treat type 2 diabetes in Japan.
  • - Revisions emphasize the importance of considering safety issues and fatty liver disease as a related health concern.
  • - The updated guidelines also highlight the role of tirzepatide in the treatment options for this condition.
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Herein, we report a new photofunctional Pb-S-based coordination polymer (CP) with the formula [Pb(ATAT)(OAc)] (ATAT = 3-amino-5-mercapto-1,2,4-triazole, OAc = acetate, ). Apart from its photoactive one-dimensional (1D) (-Pb-S-) chain, is also composed of another 1D (-Pb-O-) chain that originates from the coordination with acetate. The coordinated acetate can be exchanged with water (HO) or dimethyl sulfoxide (DMSO), leading to the formation of a or structure that exhibits a distinct change in optical properties, including a white-to-yellow color change.

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Black-spot shell disease is an unresolved disease that decreases pearl quality and threatens pearl oyster survival. In previous studies, the bacterium Tenacibaculum sp. strain Pbs-1 was isolated from diseased Akoya pearl oysters Pinctada fucata, and a rapid, specific, and sensitive loop-mediated isothermal amplification (LAMP) assay for detecting this pathogen was established.

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  • Human cytomegalovirus (HCMV) has a large linear DNA genome of about 235 kb, divided into "Long" and "Short" domains.
  • There are four distinct isomers of the HCMV genome, and identifying the junction sequences between these domains is crucial for confirming their presence, but repeat sequences complicate this process through traditional methods.
  • Long-read sequencing with the Oxford Nanopore sequencer proved effective in identifying junctions in four isomers, revealing multiple copies of a specific sequence and showcasing the complexity and diversity of the HCMV genome.
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Insulinomas are the most common functional pancreatic neuroendocrine neoplasm; when treatment is delayed, they induce hyperinsulinemic hypoglycemia, which is life-threatening. As surgical resection is the only curative treatment for insulinoma, preoperative localization is crucial; however, localization based on conventional imaging modalities such as computed tomography (CT) and magnetic resonance imaging is often inconclusive. Somatostatin receptor-targeted imaging is another option for detecting pancreatic neuroendocrine neoplasms but has low sensitivity and is not specific for insulinoma.

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  • - This study introduces a new method for creating alkenyl isothiocyanates that include bromo and isothiocyanate groups, which are useful for various chemical transformations.
  • - The research highlights the practical application of these compounds through their use in an amine sensor featuring a coumarin-attached product.
  • - This simplified synthesis method makes it easier to obtain alkenyl isothiocyanates, which are important for biological research and studies.
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Recently, zwitterions have been proposed as novel cryoprotectants. However, some cells are difficult to cryopreserve using aqueous zwitterion solutions alone. We investigated here the reason for cell damage in such cells, and it was the osmotic pressure after freeze concentration.

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Sulfur-coordinated coordination polymers (S-CPs) have unique optoelectrical properties that originate from infinite M-S bond networks. In this study, we synthesized and characterized two polymorphs of a two-dimensional (2D) Pb(II) S-CP with a formula of [Pb(tzdt)(OAc)] (Htzdt=1,3-thiazolidine-2-thione, OAc=acetate). Our findings revealed that the thermodynamic product (KGF-26) possesses quasi-2D (-Pb-S-) layers with weak nonbonded Pb-S bonds, whereas the kinetic product (KGF-27) has intrinsic 2D (-Pb-S-) layers with Pb-S bonds.

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Airborne microbes, comprising a diverse range of bacteria and fungi, are a pervasive component of the atmosphere, with concentrations typically ranging from 10 to 10 cells per cubic meter [...

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Neuroaxonal dystrophy (NAD) is a neurodegenerative disease characterized by spheroid (swollen axon) formation in the nervous system. In the present study, we focused on a newly established autosomal recessive mutant strain of F344-/ rats with hind limb gait abnormalities and ataxia from a young age. Histopathologically, a number of axonal spheroids were observed throughout the central nervous system, including the spinal cord (mainly in the dorsal cord), brain stem, and cerebellum in F344-/ rats.

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Cryopreservation techniques are valuable for the preservation of genetic properties in cells, and the development of this technology contributes to various fields. In a previous study, an isotonic freezing medium composed of poly(zwitterion) (polyZI) has been reported, which alleviates osmotic shock, unlike typical hypertonic freezing media. In this study, the primitive freezing medium composed of emerging polyZI is optimized.

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Polyamines, such as putrescine and spermidine, are pivotal in various biological processes across living organisms. Despite their significance, structurally modified polyamines offer a less-explored avenue for discovering bioactive compounds. The limitation is attributed to the synthetic difficulty of accessing functionalized polyamines.

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One-dimensional (1D) Cd(II) coordination polymers [Cd(-SPhOMe)] ( = , , and ; HSPhOMe = methoxybenzenethiol) containing inorganic 1D (-Cd-S-) chains were synthesized. Among these, the KGF-31 polymer bearing -SPhOMe featured a three-strand chain structure assembled interchain S⋯S interactions and exhibited high photoconductivity and longevity.

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Drosophila strains must be maintained by the frequent transfer of adult flies to new vials. This carries a danger of mutational deterioration and phenotypic changes. Development of an alternative method for long-term preservation without such changes is therefore imperative.

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During the long-term storage of cells, it is necessary to inhibit ice crystal formation by adding cryoprotectants. Non-cell-permeable cryoprotectants have high osmotic pressure which dehydrates cells, indirectly suppressing intracellular ice crystal formation. However, the high osmotic pressure and dehydration often damage cells.

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  • The text addresses a correction concerning an academic article identified by its DOI (Digital Object Identifier) 10.1007/s13340-022-00605-x.
  • It suggests that there were errors or inaccuracies in the original publication that needed to be rectified.
  • The correction aims to ensure the integrity and accuracy of the research presented in the article for future reference.
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Article Synopsis
  • - The study highlights that Potential Airborne Human Pathogens (PAHPs) are often overlooked in indoor air quality standards, despite their ability to cause infections, especially in vulnerable populations like immunosuppressed patients.
  • - It identifies human occupancy and ventilation systems as key factors influencing the spread of microbial aerosols, with temperature and humidity also playing significant roles in PAHP viability.
  • - The research calls for a revision of public health policies and IAQ standards to include considerations of PAHPs, emphasizing the need to address their biological risks rather than just their abundance in indoor environments.
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In a previous study, we isolated a Vibrio sp. strain MA3 and its virulence factor, a hemolysin encoded by vhe1. This strain is associated with mass mortalities of the pearl oyster Pinctada fucata.

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Pyrethrins from are natural pesticides that exhibit high knockdown and killing activities against flying insects such as disease-spreading mosquitoes. Despite the increasing demand for pyrethrins, the mechanism of pyrethrin biosynthesis remains elusive. To elucidate it, we for the first time created pyrethrin mimetic phosphonates targeting the GDSL esterase/lipase (GELP or TcGLIP) underpinning pyrethrin biosynthesis.

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Aims: Sodium-glucose co-transporter 2 (SGLT2) inhibitors reduce the risk of heart failure (HF) events regardless of diabetes status. However, factors associated with their efficacy in HF reduction remain unknown. This study aims to identify clinically relevant markers for the efficacy of SGLT2 inhibitors in HF risk reduction.

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