Facile construction of dual-response super-resolution probes for tracking organelles dynamics.

Super-resolution imaging techniques, such as structured illumination microscopy (SIM), have enabled researchers to obtain nanoscale organelle-level outputs in living systems, but they impose additional stringent requirements on fluorescence probes. However, high-performance, custom-designed SIM probes that can explain underlying biological processes remain unavailable. Herein, a customizable engineering toolkit is developed for the facile assembly of SIM probes suitable for subcellular component detection.

A near-infrared, colorimetric and ratiometric fluorescent sensor with high sensitivity to hydrogen peroxide and viscosity for solutions detection and imaging living cells.

Considering to the importance of hydrogen peroxide (HO) in a number of pathological processes and fluorescence sensor as a powerful tool for imaging and therapy of HO-related diseases, herein, a near-infrared fluorescent sensor for colorimetric and ratiometric detecting HO and viscosity, named NCR, was constructed and reported. Its long emission wavelength (670 nm) avoids the interference of biological autofluorescence. When NCR interacted with 10 equiv.

[Determination of 1, 1-dichloro-l-nitroethane in air of workplaces by gas chromatography].

Objective: To establish a gas chromatography method for detecting the concentration of 1,1-dichloro-1-nitroethane in air of workplaces.

Method: 1,1-dichloro-1-nitroethane in air of workplaces was collected by activated charcoal tube, absorbed using carbon disulfide and analyzed by Gas Chromatography (FID) with FFAP capillary column.

Results: The linear rang of 1,1-dichloro-1-nitroethane in this method was 4.

[Evaluation of urinary ortho-cresol as biological monitoring indicator of occupational exposure to toluene].

Objective: To investigate the feasibility of urinary ortho-cresol levels as a biological monitoring indicator for toluene exposure.

Methods: Establish pre-column derivation HPLC method to detect ortho-cresol levels in urine of human body. Ortho-cresol levels in urine of workers non-exposed and exposed to toluene was examined using this pre-column derivation HPLC method, and exposure assessment was conducted.