Design, synthesis of ceramide 1-phosphate analogs and their affinity for cytosolic phospholipase A as evidenced by surface plasmon resonance.

Ceramide 1-phosphate (C1P) is a lipid mediator that specifically binds and activates cytosolic phospholipase Aα (cPLAα). To elucidate the structure-activity relationship of the affinity of C1P for cPLAα in lipid environments, we prepared a series of C1P analogs containing structural modifications in the hydrophilic parts and subjected them to surface plasmon resonance (SPR). The results suggested the presence of a specific binding site for cPLAα on the amide, 3-OH and phosphate groups in C1P structure.