Extracellular production of phospholipase A2 from Streptomyces violaceoruber by recombinant Escherichia coli.

Phospholipase A(2) (PLA(2)) from Streptomyces violaceoruber was successfully produced extracellularly in an active form by using a recombinant strain of Escherichia coli. The PLA(2) gene, which was artificially synthesized with optimized codons for E. coli and fused with pelB signal sequence, was expressed in E.

The Candida glabrata putative sterol transporter gene CgAUS1 protects cells against azoles in the presence of serum.

Objectives: The uptake of endogenous sterol from serum may allow Candida glabrata to survive azole treatment. This study aims to determine the contribution of a sterol transporter that alters fluconazole sensitivity in the presence of serum.

Methods: Bioinformatic analysis predicted CgAUS1 as the C.