[Analysis of glycan ratio of Chinese hamster ovary cell-cetuximab antigen-binding segment via rapid enzyme digestion with endo---acetylglucosaminidase F].

The -glycosylation of proteins is a typical post-translational modification. Compared with other monoclonal antibodies, -glycosylation modification in cetuximab is more complicated. Because cetuximab contains two -glycosylation sites, one is located on the antigen-binding fragment (Fab) and the other is on the crystallizable fragment (Fc) of the heavy chain (HC).