Dual-functional DNAzyme powered CRISPR-Cas12a sensor for ultrasensitive and high-throughput detection of Pb in freshwater.

Freshwater lead pollution has posed severe threat to the environment and human health, underscoring the urgent necessity for accurate and user-friendly detection methods. Herein, we introduce a novel Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR-Cas) sensor for highly sensitive Pb detection. To accomplish this, we designed a dual-functional deoxyribozyme (df-DNAzyme) probe that functions as an activator for the CRISPR-Cas12a system while also recognizing Pb.

Smartphone-based surface plasmon resonance sensing platform for rapid detection of bacteria.

Bacterial infection poses severe threats to public health, and early rapid detection of the pathogen is critical for controlling bacterial infectious diseases. Current methods are commonly labor intensive, time consuming or dependent on lab-based equipment. In this study, we proposed a novel and practical method for bacterial detection based on smartphones using the surface plasmon resonance (SPR) phenomena of gold nanoparticles (AuNPs).

Rapid measurement of waterborne bacterial viability based on difunctional gold nanoprobe.

Rapid measurement of waterborne bacterial viability is crucial for ensuring the safety of public health. Herein, we proposed a colorimetric assay for rapid measurement of waterborne bacterial viability based on a difunctional gold nanoprobe (dGNP). This versatile dGNP is composed of bacteria recognizing parts and signal indicating parts, and can generate color signals while recognizing bacterial suspensions of different viabilities.

In situ detection of microbial c-type cytochrome based on intrinsic peroxidase-like activity using screen-printed carbon electrode.

C-type cytochromes (c-cyts) facilitate microbial extracellular electron transfer and play critical roles in biogeochemical cycling, bioelectricity generation and bioremediation. In this study, a simple and effective method has been developed to detect microbial c-cyts by means of peroxidase mimetic reaction on screen-printed carbon electrode (SPCE). To this end, bacteria cells were immobilized onto the working electrode surface of SPCE by a simple drop casting.

Preparation and characterization of egg yolk immunoglobulin Y specific to influenza B virus.

The aim of this study was to prepare egg yolk immunoglobulin (IgY) for use in the prevention and treatment of influenza B viral infections. Laying hens were immunized with inactivated influenza B virus (IBV), and IgY was isolated from the egg yolk by multiple polyethylene glycol (PEG) 6000 extraction and ammonium sulfate purification steps. The titers and specificity of the purified antibodies were assessed.