[Effect of collagen sponge on the healing process of alveolar fossa after tooth extraction in rats].

Purpose: To investigate the effect of collagen sponge on early bone healing process of alveolar fossa after tooth extraction in rats.

Methods: A total of 16 healthy female SD rats were selected. Animal models with tooth extraction were established.

[Expression and clinical significance of PUMA gene in salivary adenoid cystic carcinoma].

Purpose: To explore the expression of p53 up-regulated modulator of apoptosis (PUMA) gene in salivary adenoid cystic carcinoma (SACC) and its clinical significance.

Methods: The expressions of PUMA gene and protein were detected in 27 SACC tumor-adjacent tissues (group A) and 27 SACC tumor tissues (group B) with real-time quantitative PCR and Western blot. Statistical analysis was performed using SPSS 19.

[The clinical value of combining multiple features of CT in discriminating between benign and malignant lung lesions].

Objective: To develop a set of combined criteria of multiple features of chest CT for discriminating between benign and malignant lung lesions.

Methods: Patients whose chest CT showed abnormalities were recruited from the West China Hospital in March and April 2010. The patients were examined with bronchoscopy and the results of CT and pathology were compared.

[Establishment of an experimental animal model with alveolar bone defect by surgery in rats].

Purpose: To establish an animal model for experimental study of orthodontic treatment of alveolar bone defect and other relative research.

Methods: The experiment was carried out on forty SPF female Wistar rats,which were 6 weeks old. With the rats anaesthetized, one side of the alveolar bone in the maxillary first molar of the rats was removed partly.

Distribution and patterns of lymph nodes metastases and micrometastases in the mesorectum of rectal cancer.

Background And Objectives: Facts buried in the mesorectum remain to be unveiled. This study investigated the number, size, and detailed distribution of lymph nodes metastases and micrometastases within the mesorectum of rectal cancer.

Methods: Thirty-one patients who underwent total mesorectal excision (TME) were treated with lymph node revealing solution to retrieve lymph nodes, which were submitted to hematoxylin and eosin (HE) examination and immunohistochemical (IHC) staining.

Combined histology and molecular biology for diagnosis of early stage gastric MALT lymphoma.

Objective: To establish a sequential diagnostic procedure of gastric mucosa-associated lymphoid tissue (MALT) lymphoma and provide evidence for selected optimal cases to be treated in the early stage.

Methods: Thirty-one cases of gastric lymphoid hyperplasia (GLH) were selected and multiple investigations including histology, protein level, DNA and chromosome levels, combined with clinical follow-up were performed. Histological grade was according to Isaacson's criteria of GLH; CD20, UCHL-1 (CD45RO), anti-kappa (kappa), anti-lambda (lambda) and Ki-67 were used for immunohistochemical staining; semi-nested polymerase chain reaction (PCR) was used to detect IgH gene rearrangement and reverse-transcription PCR (RT-PCR) was used to detect API2-MALT1 fusion of the chromosome translocation t(11;18)(q21;q21).

[Correlation study between sex and age of patients and root resorption after orthodontic treatment].

Purpose: To investigate the relationship between root resorption related to fixed appliance and the variables including sex, age.

Methods: 44 patients were divided into four groups according to sex and age: juvenile males, juvenile females, adult males, adult females. The length of incisors was calculated according to orthopantomograph and study models of pre- and post-treatment, and the relationship between patients' sex and age and root absorption pre- and post-treatment was analyzed by paired t test.

[Hemangioma and vascular malformation of small intestine: a clinicopathologic analysis of fifty-one cases].

Objective: To investigate the clinicopathologic features of small intestinal hemangioma and vascular malformation.

Methods: A retrospective analysis was performed on 51 cases of hemangioma and vascular malformation involving the duodenum, jejunum and ileum in the past 30 years.

Results: The patient's age ranged from 1.

[Study with large slice technique and tissue microarray on lymph node involvement and micro-metastasis of rectal cancer].

Objective: To study lymph node involvement and micro-metastasis of rectal cancer with large slice technique and tissue microarray.

Methods: Large slice technique, combined with tissue microarray,was used in pathologic study of 31 patients after total mesorectal excision (TME) for rectal cancer.

Results: Nine hundred and ninety- two lymph nodes were harvested and 148 were positive.

Mesorectal spread and micrometastasis of rectal cancer studied with large slice technique and tissue microarray.

Background And Objectives: Mesorectal tissue seems to be an ideal substrate for the spreading of tumors. The aim was to study the distribution of mesorectal neoplastic foci, examine occurrence of circumferential margin involvement and investigate micrometastasis of the lymph nodes.

Methods: A large slice technique, combined with tissue microarray, was used in the pathologic study of 31 specimens operated on following the principles of total mesorectal excision (TME).

Nodal spread and micrometastasis within mesorectum.

Aim: To study the distribution of positive lymph nodes within mesorectum and to investigate the possible micrometastasis in negative lymph nodes.

Methods: Large slice technique combined with tissue microarray was used in the pathologic study of 31 specimens.

Results: A total of 992 lymph nodes were harvested and cancer metastasis was found in 148 lymph nodes.

Significance of glucocorticoid receptor expression in colonic mucosal cells of patients with ulcerative colitis.

Aim: Glucocorticoid (GC) resistant ulcerative colitis (UC) remains a serious disease and is difficult to manage. Although the molecular basis of GC insensitivity is still unknown, GC receptors (GRalpha and GRbeta) may play an important role in it. This study was aimed to investigate the relationship between the expression of GRalpha and GRbeta in colonic mucosal cells of patients with UC, the efficacy of GC therapy and the intensity of inflammation.

[Interobserver reproducibility in the pathologic diagnosis of borderline ductal proliferative breast diseases].

Objective: The aim of this study involving general pathologists was to assess interobserver reproducibility in the pathologic diagnosis of borderline ductal proliferative breast diseases.

Methods: Ten general pathologists independently reviewed 43 specimens chosen to represent the spectrum of borderline ductal proliferative breast lesions. All slides were blindly reviewed without given standardized criteria, and were classified as either mild usual hyperplasia, moderate-severe usual hyperplasia, mild atypical hyperplasia, moderate-severe atypical hyperplasia, ductal carcinoma in situ, or ductal carcinoma in situ with invasion.

Tumor micrometastases in mesorectal lymph nodes and their clinical significance in patients with rectal caner.

Aim: To investigate the number, size, and status of lymph nodes within the mesorectum and to explore the prognostic significance of lymph node micrometastases in patients with rectal cancer.

Methods: Thirty-one patients with rectal cancer undergone total mesorectal excision between October 2001 and October 2002 were included. Mesorectal nodes retrieved from the resected specimens were detected with a combination of haematoxylin and eosin (HE) staining and immunohistochemistry (IHC).

[Mesorectal spread and circumferential margin involvement of rectal cancer studied on large slice].

Objective: To provide the mode of cancer distribution in the mesorectum and circumferential resection margin.

Methods: Large slice technique was used in the pathologic study of 62 specimens operated on following the principles of total mesorectal excision (TME).

Results: More than 40% of all the observed neoplastic foci were located in the outer layer of the mesorectum in Stage III patients.

[Study on glucocorticoid receptor alpha and beta in colonic mucosal cell of patients with ulcerative colitis].

Objective: To investigate the relationship of the expression of GRalpha and GRbeta in the colonic mucosal cell of patients with ulcerative colitis to the efficacy of glucocorticoid (GC) therapy and the intensity of inflammation.

Methods: GRalpha expression and GRbeta expression in the colonic mucosal specimens were assessed by means of immunohistochemistry. Then comparative analyses were made on the GRalpha and GRbeta expression between the GC resistant group and GC sensitive group at various levels of inflammation.

Microscopic spread of low rectal cancer in regions of mesorectum: pathologic assessment with whole-mount sections.

Aim: To assess the microscopic spread of low rectal cancer in mesorectum regions to provide pathological evidence for the necessity of total mesorectal excision (TME).

Methods: A total of 62 patients with low rectal cancer underwent low anterior resection and TME, surgical specimens were sliced transversely on the serial embedded blocks at 2.5 mm interval, and stained with hematoxylin and eosin (HE).

[The early diagnosis and treatment of gastric mucosa-associated lymphoma].

Objective: To determine the general markers for the diagnosis of gastric mucosa-associated lymphoid tissue (MALT) lymphoma and select optimal cases to be interfered in early stage.

Methods: To investigate the changes of histology, Ki-67 expression, monoclonality of IgH gene rearrangement in gastric lymphoid hyperplasia (GLH) cases before and after Helicobacter pylori (Hp) eradication treatment. Histological changes were classified according to Isaacson's criteria of GLH; L26, UCHL-1, anti-Kappa, anti-Lambda and Ki-67 were applied for immunohistochemical staining; semi-nest PCR was used to detect IgH gene rearrangement.