Validation of a method for the determination of triphenylmethane dyes in trout and shrimp with superior extraction efficiency.

The described methods are able to analyse the triphenylmethane dyes malachite green (MG), crystal violet (CV) and brilliant green (BG) as well as their leuco metabolites leuco malachite green (LMG), leuco crystal violet (LCV) and leuco brilliant green (LBG) on the basis of a simple and fast extraction. The validation of the methods in two studies without and with a heated ultrasonic treatment during the extraction of fortified trout and shrimp samples was successfully performed applying an in-house validation concept. The evaluation of the relevant validation parameters, e.