Effects of microwave (2.45 GHz) irradiation on some biological characters of Salmonella typhimurium.

The present study was carried out to evaluate the effects of sub-lethal doses of microwave radiation on some biological characteristics in Salmonella typhimurium. The aim was to show the relationship between this treatment and the development of radiotolerance in this pathogen because there is a need for more information on physiological responses of pathogens to sub-lethal doses of microwave radiation. So, the bacterial strain was treated with a dose of 3600J (40-s exposure with power P=90 W) to cause cellular damage.

SeqA, the Escherichia coli origin sequestration protein, can regulate the replication of the pBR322 plasmid.

The pBR322 plasmid origin replication and oriC show similar responses to adenine methylation. Both are subject to sequestration by membrane fractions. In fact, like the host origin oriC, the RNA II promoter region of pBR322 is regulated by methylation of three GATC adenine methylation sites.

Effect of seqA mutation on Salmonella typhimurium virulence.

It is well established that lack of SeqA protein leads to reinitiation at the same origin more than once per cell cycle, in E. coli. Aberrant cell division and filament formation in SeqA-deficient strains suggest that this protein might be involved in cell cycle process other than transient inhibition of replication initiation.

Phospholipid changes in seqA and dam mutants of Escherichia coli.

SeqA and Dam proteins were known to be responsible for regulating the initiation of replication and to affect the expression of many genes and metabolisms. We have examined here the fatty acids composition and phospholipids membrane in dam and/or seqA mutants. The dam mutant showed an accumulation of the acidic phospholipids cardiolipin, whereas, the seqA mutant showed a higher proportion of phosphatidylglycerol compared with the wild-type strain.