Publications by authors named "Daehan Nam"
In the field of molecular diagnostics, the demand for multiplex detection, aimed at reducing overall analysis costs and streamlining procedures, is on the rise, prompting ongoing developments in various technologies. In this study, we developed a novel system, the split T7 promoter-based three-way junction-transcription, coupled with Cas12a/Blocker DNA (T3-CaB), for the multiplex detection of target nucleic acids. The T3-CaB system builds upon the foundation of the T3 system, generating numerous RNA transcripts upon encountering target nucleic acids.
View Article and Find Full Text PDFAptamer-Based Switching System for Communication of Non-Interacting Proteins.
Biosensors (Basel)
January 2024
Biological macromolecules, such as DNA, RNA, and proteins in living organisms, form an intricate network that plays a key role in many biological processes. Many attempts have been made to build new networks by connecting non-communicable proteins with network mediators, especially using antibodies. In this study, we devised an aptamer-based switching system that enables communication between non-interacting proteins.
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- Loop-mediated isothermal amplification (LAMP) is a popular method for DNA amplification but typically requires a high temperature of 65 °C, which can limit its application in on-site diagnostics.
- This study introduces a new method called low-temperature LAMP that operates efficiently at a more physiological temperature of 37 °C, adjusting key components in the process to maintain amplification effectiveness.
- The low-temperature LAMP method allows for easy detection of important cancer-related miRNAs without needing costly modifications, making it suitable for use in resource-limited settings.