Application of Metagenomic High-Throughput Sequencing in a Rare Case of Multisite Infection With Two Microorganisms After Total Knee Arthroplasty.

Postoperative deep infection is usually identified by microbial culture. However, frequent false-negative results have severely limited effective treatment. We report a rare case of intra-articular and paravertebral infection after total knee arthroplasty caused by and , with multiple negative microbial culture results.

Precise Patellar Tendon Insertion Protection and Osteotomy Surface Advantage of Transtibial Tuberosity-High Tibial Osteotomy.

Objective: Medial opening wedge high tibial osteotomy (HTO) is successful in the treatment of knee osteoarthritis with medial compartment stenosis and tibial varus deformity, but patella infera is the main complication. This study aims to design a new medial tibial open osteotomy scheme, transtibial tuberosity-high tibial osteotomy (TT-HTO), which can fully protect the patellar tendon insertion. In addition, the area of the osteotomy surface and wedge volume were evaluated in TT-HTO, biplanar distal tibial tuberosity osteotomy (biplanar-DTO), and uniplanar-DTO to evaluate the potential advantages of this technology in bone healing.

Light triggered nanoscale biolistics for efficient intracellular delivery of functional macromolecules in mammalian cells.

Biolistic intracellular delivery of functional macromolecules makes use of dense microparticles which are ballistically fired onto cells with a pressurized gun. While it has been used to transfect plant cells, its application to mammalian cells has met with limited success mainly due to high toxicity. Here we present a more refined nanotechnological approach to biolistic delivery with light-triggered self-assembled nanobombs (NBs) that consist of a photothermal core particle surrounded by smaller nanoprojectiles.

Biomechanical analysis of the drilling parameters for early osteonecrosis of the femoral head.

Background And Objective: Core decompression is a surgical procedure commonly used to treat the early osteonecrosis of the femoral head. However, It is not known whether different drilling parameters affect postoperative biomechanical strength. This study aimed to analyze the mechanical stability of different drilling locations and diameters of core decompression using finite element analysis.

Core-shell microparticles: From rational engineering to diverse applications.

Core-shell microparticles, composed of solid, liquid, or gas bubbles surrounded by a protective shell, are gaining considerable attention as intelligent and versatile carriers that show great potential in biomedical fields. In this review, an overview is given of recent developments in design and applications of biodegradable core-shell systems. Several emerging methodologies including self-assembly, gas-shearing, and coaxial electrospray are discussed and microfluidics technology is emphasized in detail.

Bubble Forming Films for Spatial Selective Cell Killing.

Photodynamic and photothermal cell killing at the surface of tissues finds applications in medicine. However, a lack of control over heat dissipation following a treatment with light might damage surrounding tissues. A new strategy to kill cells at the surface of tissues is reported.

A novel xanthan gum-based conductive hydrogel with excellent mechanical, biocompatible, and self-healing performances.

Tough and conductive hydrogels are promising materials for various applications. However, it remains a great challenge to develop an integrated hydrogel combining outstanding mechanical, conductive, and self-healing performances. Herein, we prepared a conductive, self-healing, and tough hydrogel by constructing synergistic multiple interaction among montmorillonite (MMT), Poly (acrylamide-co-acrylonitrile) (P(AAm-co-AN)), xanthan gum (XG) and ferric ion (Fe).

Materials and Technologies to Combat Counterfeiting of Pharmaceuticals: Current and Future Problem Tackling.

The globalization of drug trade leads to the expansion of pharmaceutical counterfeiting. The immense threat of low quality drugs to millions of patients is considered to be an under-addressed global health challenge. Analytical authentication technologies are the most effective methods to identify active pharmaceutical ingredients and impurities.

Stimuli-responsive bio-based polymeric systems and their applications.

Stimuli-responsive bio-based polymeric systems are gaining considerable attention as intelligent versatile tools that show great potential in various fields. In this review, an overview is given of recent developments of stimuli-responsive bio-based polymeric systems. The characteristics of bio-based polymers in different applications are discussed and the superiority of these advanced stimuli-responsive bio-based polymeric systems is highlighted.

Green electrospun and crosslinked poly(vinyl alcohol)/poly(acrylic acid) composite membranes for antibacterial effective air filtration.

Air pollution has become a major environmental concern given the ever increasing levels of particulate matter (PM) and the increased in treatment-resistant bacterial and viral strains. Major efforts are therefore required into the development of air filtration and purification technology as well as novel, alternative antiviral and antibacterial treatment modalities. Here, we report an environmentally friendly method for the generation of multifunctional poly(vinyl alcohol)/poly(acrylic acid) (PVA-PAA) composite membranes via green electrospinning and thermal crosslinking.

Synthesis and Evaluation of New Quinoxaline Derivatives of Dehydroabietic Acid as Potential Antitumor Agents.

A series of new quinoxaline derivatives of dehydroabietic acid (DAA) were designed and synthesized as potential antitumor agents. Their structures were characterized by IR, ¹H-NMR, C-NMR, and MS spectra and elemental analyses. All the new compounds were screened for their in vitro antiproliferative activities against three human cancer cell lines (MCF-7, SMMC-7721 and HeLa) and noncancerous human hepatocyte cells (LO2).

Synthesis and in vitro cytotoxic evaluation of new 1H-benzo[d]imidazole derivatives of dehydroabietic acid.

A series of new 1H-benzo[d]imidazole derivatives of dehydroabietic acid were designed and synthesized as potent antitumor agents. Structures of the target molecules were characterized using MS, IR, H NMR, C NMR and elemental analyses. In the in vitro cytotoxic assay, most compounds showed significant cytotoxic activities against two hepatocarcinoma cells (SMMC-7721 and HepG2) and reduced cytotoxicity against noncancerous human hepatocyte (LO2).

pH responsive polyurethane (core) and cellulose acetate phthalate (shell) electrospun fibers for intravaginal drug delivery.

In this study we present the use of co-axial electrospinning to produce core-shell composite micro-/nano- fibers of polyurethane (PU) and cellulose acetate phthalate (CAP). The designed fibers possess enhanced mechanical properties with a tensile strength of 13.27±2.

A novel preparation method of paclitaxcel-loaded folate-modified chitosan microparticles and in vitro evaluation.

A new chitosan microparticles loading paclitaxel (PTX) for application as an oral delivery system were developed using a novel double emulsion crosslinking method. To improve the targeted effect, folic acid (FA) was introduced onto the surface of microparticles using chemical method. The method was based on Schiff reaction between amino group of chitosan and carboxyl group of FA, and folate-chitosan (FA-CS) conjugate was characterized using infrared spectrum analysis (FT-IR), and the microparticles were named as FA-CS-PTX/MPs.