NSUN2-mediated mC modification drives alternative splicing reprogramming and promotes multidrug resistance in anaplastic thyroid cancer through the NSUN2/SRSF6/UAP1 signaling axis.

Anaplastic thyroid carcinoma (ATC) is an extraordinarily aggressive form of thyroid cancer, frequently presenting with locally advanced infiltration or distant metastases at the time of initial diagnosis, thus missing the optimal window for surgical intervention. Consequently, systemic chemotherapy and targeted therapies are vital for improving the prognosis of ATC. However, ATC exhibits significant resistance to conventional treatments, highlighting the need to elucidate the biological mechanisms underlying this drug resistance and identify novel therapeutic targets to overcome it.

Theoretical Studies on the Competing Mechanism and Origin of Diastereoselectivity of NHC-Catalyzed Intramolecular [3 + 2] Annulations of Ynals.

Chiral tricyclic 6,5,5-fused rings exhibit structural diversity and possess important biological activities in the synthesis of natural products. However, predicting the possible mechanism and origin of stereoselectivity in these reactions remains a challenge. In this article, we conducted a theoretical investigation into the NHC-catalyzed intramolecular [3 + 2] annulations of ynals to generate tricyclic 6,5,5-fused rings.

Safety and immunogenicity of hepatitis E vaccine in compensated liver cirrhosis with chronic hepatitis B.

Objectives: Hepatitis E virus (HEV) is a global health concern that causes acute hepatitis with severe consequences, particularly in patients with chronic liver disease. Despite the availability of a recombinant hepatitis E vaccine (Hecolin), its safety and immunogenicity in patients with pre-existing liver cirrhosis remain uncertain. This study aimed to assess the safety and immunogenicity of Hecolin in individuals with compensated liver cirrhosis who have chronic hepatitis B (CHB).

Cysteines shape antibody battles for HIV-1 Env.

In this issue of Cell Host & Microbe, Hesselman et al. investigate the relationship between the presence of non-canonical cysteine residues in HIV-1 V1 region of the Envelope glycoprotein and the development of neutralization breadth through population-based analyses.

Long-Day Photoperiod Improves the Growth and Muscle Quality of Grass Carp ().

To investigate the effects of photoperiods on the growth and muscle quality indicators of grass carp (), 225 fish (109.65 ± 3.62 g) were randomly assigned into five different photoperiod groups (0L:24D, 8L:16D, 12L:12D, 16L:8D, and 24L:0D).

Deposited dead algae influence the microbial communities and functional potentials on the surface sediment in eutrophic shallow lakes.

Dead algae deposition will change the nutrient transformation on the sediment-water interface. However, the key factors that drive nutrient turnover, particularly the influence of sediment microbiota, remain poorly understood. As a result, this study conducted an 80-day simulated incubation to investigate the effect of different deposition of death algae on microbial communities and functional potentials in sediments.

Biological VFAs production from proteinaceous wastewater varied with protein type: The role of protein exposed enzyme cleavage sites and hydrolysates biotransformation capacity.

Proteinaceous wastewater contains various proteins, which can be valorized to biobased volatile fatty acids (VFAs), important substrates for the synthesis of biodegradable plastics, biodiesel, bioelectricity, etc., but the influence of protein type on VFAs has never been documented. It was found that among the five proteinaceous wastewater proteins investigated, ovalbumin and casein produced the most and the least VFAs, respectively.

Risk Assessment of Cyclaniliprole Resistance in Peach-Potato Aphid Myzus persicae: Laboratory Selection, Inheritance, and Cross-Resistance Patterns.

Cyclaniliprole, a type of the third-generation anthranilic diamide insecticide, was mainly used for management of various pests. Myzus persicae (Hemiptera: Aphididae), known as the peach-potato aphid, is an economically essential pest with worldwide distribution. However, the risk assessment of cyclaniliprole in M.

Multiple gene-deletion vaccinia virus Tiantan strain against mpox.

Monkeypox virus (MPXV) is an important zoonotic pathogenic virus, which poses serious threats to public health. MPXV infection can be prevented by immunization against the variola virus. Because of the safety risks and side effects of vaccination with live vaccinia virus (VACV) strain Tian Tan (VTT), we constructed two gene-deleted VTT recombinants (TTVAC7 and TTVC5).

The MC-LR induced neuroinflammation and the disorders of neurotransmitter system in zebrafish (Danio rerio): Oxidative stress as a key.

Microcystin-leucine-arginine (MC-LR) has been shown to induce neuroinflammation and disrupt neurotransmitter system. However, little is known about the mechanism of toxicity. In this study, male adult zebrafish (Danio rerio) were exposed to MC-LR at concentrations of 0, 0.

Multifocality increases the risk of central compartment lymph node metastasis but is not related to the risk of recurrence and death in papillary thyroid carcinoma.

Background: Multifocality is a distinctive feature of papillary thyroid carcinoma (PTC); however, the biological behavior of PTC and its optimal management strategy remain unclear. The aim of this study was to analyze the clinical features and prognostic differences of multifocal papillary carcinoma and to guide the precise treatment of multifocal papillary carcinoma.

Methods: The medical records of 1,139 patients with PTC who had undergone total or hemi-thyroidectomy between April and October 2013 at the Tianjin Medical University Cancer Institute and Hospital were reviewed.

Influence of microbial agents-loaded biochar on bacterial community assembly and heavy metals morphology in sewage sludge compost: Insights from community stability and complexity.

Enhancing the passivation of heavy metals and increasing organic matter content during the composting of sewage sludge poses significant challenges for maximizing its utilization value. Results indicated that in the control, biochar, microbial agents and microbial agents-loaded biochar (BCLMA) groups, BCLMA addition led to a higher composting temperature, with increases of 17-62% in humic acid, 25-73% in germination index, and 30-35% in organic matter consumption. And the residual fraction of Cu, Zn, Cr and Cd were increased by 30%, 12%, 22% and 17%, respectively.

Chemical cross-linking facilitates antigen uptake and presentation and provides improved protection from Mpox with a dual-antigen subunit vaccine.

Antigen uptake, processing, and presentation are crucial for the immune responses of protein-based vaccines. Herein, we introduced a reversible chemical cross-linking strategy to engineer protein antigens, which can be tracelessly removed upon antigen-presenting cell (APC) uptake and cellular reduction. The chemically cross-linked antigen proteins presented significantly enhanced uptake and epitope presentation by APC.

Identification of quantitative trait loci and candidate genes associated with growth curve parameters in chinese wenshang barred chickens.

The growth curve is a vital instrument for assessing and forecasting weight and developmental shifts in livestock and poultry, which reflects the changes of bodyweight traits with time and plays a key role in guiding breeding and production approaches. This study performed a genome-wide association study (GWAS) for growth curve parameters generated by nonlinear models which fit original weight-age records, to discover the SNPs and candidate genes correlated with growth traits. Data from 362 Chinese Wenshang Barred Chickens weighed at the age of 0, 2, 4, 6, 8, 10, 12, 14, 16, and 18 weeks were used to fit the Gompertz, Logistic, and von Bertalanffy growth models.

Sesamin Alleviates Allergen-Induced Diarrhea by Restoring Gut Microbiota Composition and Intestinal Barrier Function.

Food allergens are the key triggers of allergic diarrhea, causing damage to the immune-rich ileum. This weakens the mucosal barrier and tight junctions, increases intestinal permeability, and exacerbates allergen exposure, thereby worsening the condition. Sesamin, a natural lignan isolated from sesame seed, has shown potential in regulating immune responses, but its effects on intestinal health remain unclear.

Comparative Transcriptome Analysis Reveals Mechanisms of Differential Salinity Tolerance Between and .

Background: and have obvious morphological features and strongly tolerate saline-alkali environments. However, the mechanisms that lead to the differences in saline-alkali tolerance between them remain unclear.

Methods: In this study, we employed comparative transcriptome analysis to investigate and under saline-alkali stress.

Comparative Analysis of Intestinal Microbiota in Wild, Domesticated, and Cultured .

The impact of the living environments on the intestinal microbiota of was studied by analyzing intestinal microbiota diversity, composition, and potential function among wild, domesticated, and cultured groups. The results showed that the fish living in the wild environment exhibited the highest alpha diversity of intestinal microbiota. Intestinal microbial communities in the three groups clustered by living environment, with domesticated and cultured groups showing higher similarity.

Vertical directional coupling based grating emission engineering for optical phased arrays: publisher's note.

This publisher's note contains a correction to Opt. Lett.49, 3102 (2024)10.

Microcystin-LR induces fatty liver metabolic disease in zebrafish through the PPARα-NOD1 pathway: In vivo, in vitro, and in silico investigations.

Hepatic lipid metabolism dysfunction caused by cyanobacteria bloom-released microcystin-LR (MC-LR) contributes to the development of nonalcoholic fatty liver disease and nonalcoholic steatohepatitis (NASH), thereby severely impacting the health and safety of animals and humans. In this study, the effects and mechanisms of different environmental concentrations of MC-LR (0, 0.1, 1, and 10 μg/L) on fatty liver metabolic disease in zebrafish were investigated using in vivo, in vitro, and in silico models.

Robust, Antifouling, and Hydrophilic Particle-Based Double-Network Hydrogel-PVDF Interpenetrating Microfiltration Membrane.

Poly(vinylidene fluoride) (PVDF) membranes with highly hydrophilic and antifouling properties are desirable for oily wastewater treatment. Herein, we report (1) a strategy of bulk modification of PVDF by integration of PVDF and a particle-based double-network (PDN) hydrogel, poly-2-acrylamido-2-methylpropanesulfonate/polyacrylamide (PAMPS/PAAm), via a strong PDN and PVDF interpenetrating polymer network (PDN-PVDF IPN) to obtain a PVDF/PDN solution and (2) the subsequent casting of it into a microfiltration membrane via spray-assisted non-solvent-induced phase separation (SANIPS). The IPN structure modulates the surface segregation behavior of the highly hydrophilic and robust PDN hydrogel in the process of SANIPS, endowing the resulting PVDF/PDN membrane with excellent bulk mechanical properties and much enhanced wettability and thereby high oil/water emulsion separation efficiency and antifouling performance.

Characterization of dipyridamole as a novel ferroptosis inhibitor and its therapeutic potential in acute respiratory distress syndrome management.

: Ferroptosis in lung epithelium and endothelium contributes to the pathogenesis of acute respiratory distress syndrome (ARDS), a critical and often fatal condition marked by acute inflammation and elevated pulmonary vascular permeability. Despite this, there are currently no FDA-approved therapeutics specifically targeting ferroptosis for ARDS management. : A screening of 259 FDA-approved drugs was conducted to identify an effective ferroptosis inhibitor in pulmonary epithelial and endothelial cells.

Glycogen-accumulating organisms promote phosphate recovery from wastewater by pilot-scale biofilm sequencing batch reactor: Performance and mechanism.

A high phosphate (P) recovery concentration was achieved in pilot-scale biofilm sequencing batch reactor (BSBR) with a low carbon source (C) cost. Especially, a high-abundance glycogen-accumulating organisms (GAOs) (13.93-31.