[Narrowing and sliding genioplasty procedure combined with mandibular outer cortex ostectomy technique to correct square jaw in short face].

Objective: To evaluate the the feasibility and effectiveness of narrowing and sliding genioplasty combined with mandibular outer cortex ostectomy technique to reshape a square jaw in short face.

Methods: From July 2005 to October 2009, a total of 57 patients received narrowing and sliding genioplasty combined with mandibular outer cortex ostectomy procedure to correct square jaw in short face. All the patients had standard frontal and lateral cephalometric radiographs, panoramic radiographs, and were photographed preoperatively and postoperatively to assess their face contour.

[Correction of low angel-square jaw with mandibular "V-Line" ostectomy combined with mandibular outer cortex ostectomy].

Objective: To evaluate the feasibility of mandibular "V-Line" osteotomy combined with mandibular outer cortex osteotomy for correction of low angle-square jaw.

Methods: From Jul. 2005 to Nov.

Matrix metalloproteinase and its inhibitor in temporomandibular joint osteoarthrosis after indirect trauma in young goats.

Our aim was to examine the change in expression of matrix metalloproteinases (MMP-13), matrix metalloproteinases-3 (MMP-3), and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) in the articular cartilage of goats with experimentally-induced osteoarthrosis of the temporomandibular joint (TMJ) at various times. Osteoarthrosis was induced in 20 goats in the bilateral TMJ and 5 goats acted as controls. There were 5 goats in each group, and a group was killed at 7 days, and 1, 3, and 6 months postoperatively.

[Influence of thermochemotherapy on the activity of cytotoxic T lymphocyte in oral maxillofacial cancer patients].

Objective: To study the influence of thermochemotherapy on the activity of cytotoxic T lymphocyte (CTL) in peripheral blood of patients with oral maxillofacial cancer.

Methods: Twenty-one subjects with oral maxillofacial cancer were treated by thermochemotherapy, and the activity of CTL in peripheral blood was analyzed.

Results: Thermochemotherapy can obviously enhance the activity of CTL (P<0.

[Induction of drug resistance in Tca8113 cell line by exposing to chemotherapy drug].

Objective: To study the drug resistance changes in Tca8113 cell lines by exposing to carboplatin.

Methods: The concentration of carboplatin added to Tca8113 cells was increased gradually and continually, which was to induce the carhoplatin-resistance in Tca8113 cells. The sensibility to drugs of the cells was analyzed by MTT method.

[Expression of inducible nitric oxide synthase in cartilage in progression of temporomandibular joint osteoarthritis].

Objective: To study the expression of inducible nitric oxide synthase (iNOS) in cartilage of temporomandibular joint osteoarthriti (TMJOA), and to evaluate the role of iNOS in the progression of TMJOA.

Methods: The goats were used and TMJOA was induced by injection of collagenase in upper joint space. The joints were obtained and were investigated by using immunohistochemistry at 2, 4, 12, 24 weeks after injection.

[Study of reconstruction of mandibular condyle by transport distraction osteogenesis in monkeys].

Objective: To evaluate the applicability and effectiveness of reconstruction of mandibular condyle by transport distraction osteogenesis in an animal model of rhesus monkeys.

Methods: Six adult rhesus monkeys were used in this study. Bilateral condyles and articular discs were extirpated, and the technique of transport distraction osteogenesis was performed to reconstruct the condyle.

[Effect of hyperthermia on multidrug resistance of tongue squamous cell carcinoma cell line (Tca8113) and its multidrug resistance cell line Tca8113/CBDEA].

Objective: To study the effect of hyperthermia on expressions of multidrug resistance (MDR) genes in tongue squamous cell carcinoma cell line Tca8113 and its MDR cell line Tca8113/CBDEA and intracellular ADM concentration.

Methods: Cell lines were hyperthermia-treated (42 degrees C for 0.5 h), 4 h and 24 h later real-time quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) detected expressions of MDR relative genes (MDR1, MRPI, GST-pi) and HTS 7000 Plus Bio Assay Reader measured intracellular ADM concentration.

The role of bone morphogenetic protein-2 in vivo in regeneration of peripheral nerves.

We investigated the effects of bone morphogenetic protein-2 (BMP-2) and some other BMPs on regeneration of peripheral motor nerves in vivo. The facial nerves of 24 New Zealand rabbits were crushed to examine a series of retrograde changes in the facial nuclei and axons, in what has been called the "axon reaction". The facial nerves of the experimental group were treated with epineurial coaptation and BMP-2 after the injury.

[Influence of the expression of heat shock protein 70 in maxillofacial squamous cell carcinoma by thermochemotherapy].

Objective: To study the influence of thermochemotheraphy on the expression of HSP70 in maxillofacial squamous cell carcinoma.

Methods: 12 patients were treated with thermochemotheraphy twice a week, altogether 10 times. After 8 mg of Pingyangmycin infused, the patients were treated with microwave hyperthermia at 43 degrees C for 40 min.

[Experimental study on the microcosmic changes of compressed premaxillary suture with laser scanning confocal microscopy].

Objective: The aim of this experiment is to know whether active orthopedic appliance is rationale to do presurgical treatment and seek some regularities.

Methods: Laser scanning confocal microscopy and triple fluorochrome labeling in bone were used to observe and study detailed changes occurred in compressed suture systematically.

Results: Widened suture and broken fibres between the adjoining bones was observed at the 3rd week postoperatively.

[Effect of orthopedic premaxillary retrution on craniofacial development in animal model].

Objective: To observe the whole process of cranio-maxillofacial development of an animal model with bilateral premaxillary clefts, after being exerted with premaxillary retro-pressing forces.

Methods: Experimental rabbits with premaxillary clefts and without clefts were exerted with retro-pressing forces respectively. Roentgenographic cephalometric analysis and different investigations of premaxillary suture were performed during the whole craniofacial development.

[Effect of anti-human VEGF monoclonal antibody on VEGF level in serum of nude mice transplanted buccal carcinoma: a quantitative study].

Objective: Quantitative study of the effect of anti-human VEGF mAb E11 to VEGF level in serum of nude mice transplanted buccal carcinoma.

Methods: E11 was administered into BALB/c nu/nu mice which were transplanted human buccal carcinoma. The saline was administrated as negative control.

[Combining of TNP-470 and 5-Fu in inhibition of adenoid cystic carcinoma in nude mice model].

Objective: To study the effect of angiogenesis inhibitor and its combine with chemical drug in suppressing the growth of adenoid cystic carcinoma (ACC).

Methods: Acc-M cells were inoculated subcutaneous into BABL/C nu/nu mice. The mice were divided into control, different dose of TNP-470 treatment groups, 5-Fu treatment group and TNP-470 plus 5-Fu treatment group.

[Recombinant human BMP-2 accelerates bone formation of mandibular distraction osteogenesis in rabbits].

Objective: To study the effects of rhBMP-2 on bone formation of mandibular distraction osteogenesis in rabbits.

Methods: Bilateral mandibular osteotomies were performed in 12 mature rabbits. 5 mg rhBMP-2 with the collagen carrier was implanted in the osteotomy site of one side of the mandibles.

[Rhabdomysarcoma and combined treatment in oral and maxillofacial regions].

Objective: To study the treatment outcome of rhabdomysarcoma(RMS).

Methods: 74 cases of RMS with definite pathologic diagnosis treated in our department during the past 20 years were investigated. The relationship between the therapy and prognosis was analyzed.

[Detection of P-glycoprotein and glutathine S-transferase in mucoepidermoid carcinoma of salivary gland].

Objective: The aim of this study was to investigate the mechanism(MDR) of multidrug resistance(MDR) of mucoepidermoid carcinoma in salivary gland.

Methods: 40 cases of mucoepidermoid carcinoma in salivary gland were examined the MDR gene product P-glycoprotein using a monoclonal antibody JSB-1. And 10 of them were also investigated by detecting the expression of GST-pi.

[Expression and implication of Pgp, MRP, LRP, GST-pi, Topo II alpha in tongue squamous cell carcinoma].

Objective: To explore the correlation of chemotherapy efficacy in tongue squamous cell carcinoma(SCC) with expression level of P-glycoprotein(Pgp), multidrug resistance-associated protein (MRP), lung resistance-related protein (LRP), glutathiones-tranferase (GST-pi), DNA topo-isomerase II alpha (Topo II alpha).

Methods: The expression patterns of Pgp, MRP, LRP, GST-pi and Topo II alpha in 40 patients (pre and post-chemotherapy, respectively) with tongue SCC were examined by immunohistochemically labelled streptavidin bioein method (LsAB).

Results: The expression ratios of Pgp, MRP, LRP, GST-pi and Topo II alpha in pre-chemotherapy cases were 47.

[The effects of L-NMMA on experimental temporomandibular joint osteoarthrosis in goats].

Objective: To investigate the effects of repeated injections of L-NMMA on a goat model with osteoarthrotic temporomandibular joint (TMJ) disease.

Methods: Eight goats were selected in this study. Bilateral TMJ osteoarthrosis (OA) was induced by injecting 0.

[Mode of new bone formation following distraction osteogenesis in mandibular lengthening].

Objective: To investigate the mode and influential factor of new bone formation following distraction osteogenesis in mandibular lengthening.

Methods: Corticotomy was performed on bilateral mandibles in twelve adult male goats. A custom-made distractor was used to lengthen the mandible at a rate of 1 mm/day for 10 days (total 10 mm elongation).